Anti-CD14抗体[2D-15C, FMC-32] (ab63319)

概述

  • 产品名称
    Anti-CD14抗体[2D-15C, FMC-32]
    参阅全部 CD14 一抗
  • 描述
    小鼠单克隆抗体[2D-15C, FMC-32] to CD14
  • 特异性
    The clone number has been updated from (2Q1233) to (2D-15C, FMC-32) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human peripheral blood mononuclear cells.

  • 阳性对照
    • Peripheral blood monocytes.
  • 常规说明
    Though we have received a positive Abreview for this antibody in IHC-P, we have also received many negative reports in IHC-P and so we can no longer guarantee this antibody in this application. We appreciate any further feedback from users in this regard.


    ab63319 reacts with peripheral blood monocytes and weakly with granulocytes. It is negative to platelets, erythrocytes and lymphocytes. It is sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). It is negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL).

性能

应用

Our Abpromise guarantee covers the use of ab63319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/25. Dilute with isotonic buffer. Use 50ul per 1x106 peripheral blood mononuclear cells (PBMC) in 100ul buffer.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/25.
IHC-Fr 1/25. We recommend acetone fixation.

靶标

  • 功能
    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • 组织特异性
    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • 序列相似性
    Contains 11 LRR (leucine-rich) repeats.
  • 翻译后修饰
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [2D-15C, FMC-32] 图像

  • ab63319 staining CD14 in human tonsil tissue sections by IHC-Fr (formaldehyde-fixed frozen sections). Tissue samples were fixed with formaldehyde; antigen retrival was by heat mediation in EDTA (pH8) for 20 minutes at 100°C. The sample was incubated with primary antibody (1/300) at 25°C for 20 minutes. An undiluted HRP-conjugated Goat polyclonal to mouse/rabbit IgG was used as secondary antibody.

    See Abreview

Anti-CD14 antibody [2D-15C, FMC-32] (ab63319)参考文献

This product has been referenced in:
  • Erikson E  et al. In vivo expression profile of the antiviral restriction factor and tumor-targeting antigen CD317/BST-2/HM1.24/tetherin in humans. Proc Natl Acad Sci U S A 108:13688-93 (2011). Flow Cyt ; Human . Read more (PubMed: 21808013) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (Cardiac tissue)
Permeabilization
No
Specification
Cardiac tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Jun 08 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Peripheral macrophages)
Specification
Peripheral macrophages
Preparation
Cell harvesting/tissue preparation method: Ficoll gradient separation/adhesion
Sample buffer: PBS
Permeabilization
No
Gating Strategy
FL1
Username

Abcam user community

Verified customer

提交于 Mar 18 2013

According to our records, ab39256, ab63319, ab77258, ab86673, ab103674 was proving difficult to use in [APPL] and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more detail...

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Thank you for your email.

We will look forward to receive the images. On the optimization note we strongly recommend to include the blocking step in protocol; please try 5%BSA or 5-10% normal serum for 1-2 hours at room temperature with specie...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

-...

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Thank you for your email.

I am sorry this product did not perform in IHC-P as originally stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx wi...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 100C, 20 minutes
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Dec 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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