Anti-CD133抗体- Stem Cell Marker (ab19898)
Key features and details
- Rabbit polyclonal to CD133 - Stem Cell Marker
- Suitable for: ICC, ICC/IF, Flow Cyt, IHC-P, IHC-Fr, IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD133抗体- Stem Cell Marker
参阅全部 CD133 一抗 -
描述
兔多克隆抗体to CD133 - Stem Cell Marker -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab284389.
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经测试应用
适用于: ICC, ICC/IF, Flow Cyt, IHC-P, IHC-Fr, IP, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human CD133 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: O43490 -
阳性对照
- WB: Caco 2, HeLa and human embryonic stem cell lysates. ICC/IF: Caco-2 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19898于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC | (1) |
Use at an assay dependent concentration.
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ICC/IF | (6) |
Use at an assay dependent concentration.
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Flow Cyt | (3) |
Use at an assay dependent concentration.
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IHC-P | (11) |
Use at an assay dependent concentration.
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IHC-Fr | (4) |
Use at an assay dependent concentration. PubMed: 23769181
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IP |
1/30.
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WB | (2) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa).
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说明 |
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ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 23769181 |
IP
1/30. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa). |
靶标
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功能
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). -
组织特异性
Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). -
疾病相关
Retinitis pigmentosa 41
Cone-rod dystrophy 12
Stargardt disease 4
Retinal macular dystrophy 2 -
序列相似性
Belongs to the prominin family. -
翻译后修饰
Isoform 1 and isoform 2 are glycosylated.
Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. -
细胞定位
Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. - Information by UniProt
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数据库链接
- Entrez Gene: 8842 Human
- Entrez Gene: 19126 Mouse
- Entrez Gene: 60357 Rat
- Omim: 604365 Human
- SwissProt: O43490 Human
- SwissProt: O54990 Mouse
- Unigene: 614734 Human
- Unigene: 6250 Mouse
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别名
- AC133 antibody
- Antigen AC133 antibody
- CD133 antibody
see all
图片
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All lanes : Anti-CD133 antibody - Stem Cell Marker (ab19898) at 1 µg/ml
Lane 1 : Caco 2 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDaLanes 1 - 2: Merged signal (red and green). Green - ab19898 observed at 97 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab19898 was shown to react with CD133 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab19898 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab19898 staining CD133 in Caco-2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab19898 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor®594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunoprecipitation side-by-side comparison with the recombinant multiclonal antibody ab278053
This immunoprecipitation image is a comparison between ab19898 and the alternative recombinant multiclonal antibody ab278053.
Left side - Recombinant multiclonal to CD133 - ab278053
CD133 was immunoprecipitated from 0.35 mg Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab278053 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278053 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Caco-2 whole cell lysate 10 µg.
Lane 2: ab278053 IP in Caco-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278053 in Caco-2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Right side - Polyclonal antibody to [CD133] - ab19898
Same testing conditions as ab278053.
Why choose a recombinant antibody?
Research with confidence - consistent and reproducible results with every batch
Long-term and scalable supply - powered by recombinant technology for fast production
Success from the first experiment - confirmed specificity through extensive validation
Ethical standards compliant - production is animal-free -
Western blot side-by-side comparison with the recombinant multiclonal antibody ab278053
This Western blot image is a comparison between ab19898 and the alternative recombinant multiclonal antibody ab278053.
Left side - Recombinant multiclonal to CD133 - ab278053
All lanes : Anti-CD133 antibody [RM1002] (ab278053) at 1/1000 dilution
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 97 kDa
Observed band size: 120 kDa
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds.
Right side - Polyclonal antibody to [CD133] - ab19898
Same testing conditions as ab278053.
Why choose a recombinant antibody?
Research with confidence - consistent and reproducible results with every batch
Long-term and scalable supply - powered by recombinant technology for fast production
Success from the first experiment - confirmed specificity through extensive validation
Ethical standards compliant - production is animal-free -
All lanes : Anti-CD133 antibody - Stem Cell Marker (ab19898) at 1 µg/ml
Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 2 :HT-29 whole cell lysate (ab3952)
Lane 3 : Caco-2 whole cell lysate (ab3950)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
ab19898 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
All lanes : Anti-CD133 antibody - Stem Cell Marker (ab19898) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Human Embryonic Stem Cell Lysate with CD133 peptide (ab20651) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 97 kDaAnti-CD133 antibody ab19898 detects a band corresponding to the expected size of CD133 in Human Embryonic Stem Cell Lysate using Western Blotting. It is likely that the band runs higher than the predicted MW of CD133 due to glycosylation of CD133. In mouse neural stem cell and mouse embryonic stem cell lysate this ~110 kDa band was not detected but a reproducible band of ~30 kDa was seen. This could represent a cleavage product but it may also be that the ~30 kDa band represents non-specific binding by ab19898. However, ab19898 has been shown to recognise mouse neural stem cells using ICC. Blocking using the immunising peptide removed both of these bands.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (245)
ab19898 被引用在 245 文献中.
- Riva M et al. Radiation dose-escalation and dose-fractionation modulate the immune microenvironment, cancer stem cells and vasculature in experimental high-grade gliomas. J Neurosurg Sci 67:55-65 (2023). PubMed: 33056947
- Majc B et al. Upregulation of Cathepsin X in Glioblastoma: Interplay with γ-Enolase and the Effects of Selective Cathepsin X Inhibitors. Int J Mol Sci 23:N/A (2022). PubMed: 35163706
- Ding C et al. Short-read and long-read full-length transcriptome of mouse neural stem cells across neurodevelopmental stages. Sci Data 9:69 (2022). PubMed: 35236860
- Park Y et al. Impact of Exogenous Treatment with Histidine on Hepatocellular Carcinoma Cells. Cancers (Basel) 14:N/A (2022). PubMed: 35267513
- Gasmi I et al. Interleukin-17 programs liver progenitor cell transformation into cancer stem cells through miR-122 downregulation with increased risk of primary liver cancer initiation. Int J Biol Sci 18:1944-1960 (2022). PubMed: 35342340