Anti-CD130 (gp130)抗体(ab87969)

概述

  • 产品名称
    Anti-CD130 (gp130)抗体
    参阅全部 CD130 (gp130) 一抗
  • 描述
    小鼠单克隆抗体to CD130 (gp130)
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ELLDPCGYIS PESPVVQLHS NFTAVCVLKE KCMDYFHVNA NYIVWKTNHF TIPKEQYTII NRTASSVTFT DIASLNIQLT CNILTFGQLE QNVYGITIIS, corresponding to amino acids 23-122 of human CD130 (gp130) protein (NP_002175) with a proprietary tag.

  • 阳性对照
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab87969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 103 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
  • 组织特异性
    Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
  • 序列相似性
    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 结构域
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 翻译后修饰
    Phosphorylation of Ser-782 down-regulates cell surface expression.
    Heavily N-glycosylated.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD130 antibody
    • CD130 antigen antibody
    • CDw130 antibody
    • gp130 antibody
    • GP130 RAPS antibody
    • IL 6R beta antibody
    • IL-6 receptor subunit beta antibody
    • IL-6R subunit beta antibody
    • IL-6R-beta antibody
    • IL-6RB antibody
    • IL6 ST antibody
    • IL6RB_HUMAN antibody
    • IL6ST antibody
    • Interleukin 6 receptor subunit beta antibody
    • Interleukin receptor beta chain antibody
    • Interleukin-6 receptor subunit beta antibody
    • Interleukin-6 signal transducer antibody
    • Membrane glycoprotein 130 antibody
    • Membrane glycoprotein gp130 antibody
    • Oncostatin M receptor antibody
    • Oncostatin M receptor alpha subunit antibody
    • Oncostatin-M receptor subunit alpha antibody
    see all

图片

  • Anti-CD130 (gp130) antibody (ab87969) at 1 µg/ml + recombinant protein fragment (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 103 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-CD130 (gp130) antibody (ab87969) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 103 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,28 kDa,32 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab87969 has not yet been referenced specifically in any publications.

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