RabMAb

Anti-CD13抗体[EPR4059] (ab108382)

概述

  • 产品名称Anti-CD13抗体[EPR4059]
    参阅全部 CD13 一抗
  • 描述
    兔单克隆抗体[EPR4059] to CD13
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human CD13.

  • 阳性对照
    • THP-1, U937, and A375 cell lysates, Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 110 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • 组织特异性Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • 序列相似性Belongs to the peptidase M1 family.
    • 结构域Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • 翻译后修饰Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • 细胞定位Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    Anti-CD13 antibody [EPR4059] 图像

    • ab108382 staining CD13 in human kidney tissue sections by Immunohistochemistry (Formaldehyde/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% tween-20 and blocked for 60 minutes at 25°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody at a dilution of 1/400 for 1 hour at 25°C. An Alexa Flour® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1200) was used as the secondary antibody.

    • All lanes : Anti-CD13 antibody [EPR4059] (ab108382) at 1/1000 dilution

      Lane 1 : THP-1 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 110 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108382 at a dilution of 1/100.
    • ab108382 showing positive staining in Prostatic carcinoma tissue.

    • ab108382 showing positive staining in Normal liver tissue.

    • ab108382 showing positive staining in Ovarian carcinoma tissue.

    • ab108382 showing positive staining in Hepatocellular carcinoma tissue.

    • ab108382 showing positive staining in Normal breast tissue.

    • ab108382 showing positive staining in Normal tonsil tissue.

    Anti-CD13 antibody [EPR4059] (ab108382)参考文献

    ab108382 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (adult kidney)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
    Permeabilization Yes - 0.05% tween-20
    Specification adult kidney
    Blocking step commercial - Invitrogen's CAS-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative Formaldehyde
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    提交于 Dec 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"