Anti-CD13抗体[EPR4058] (ab108310)


  • 产品名称Anti-CD13抗体[EPR4058]
    参阅全部 CD13 一抗
  • 描述
    兔单克隆抗体[EPR4058] to CD13
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in human CD13

  • 阳性对照
    • THP-1 cell lysate, human fetal liver lysate, human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-CD13 antibody (Biotin) [EPR4058] (ab197630)

    Anti-CD13 antibody (HRP) [EPR4058] (ab196474)



Our Abpromise guarantee covers the use of ab108310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • 组织特异性Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • 序列相似性Belongs to the peptidase M1 family.
    • 结构域Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • 翻译后修饰Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • 细胞定位Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    Anti-CD13 antibody [EPR4058] 图像

    • All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/1000 dilution

      Lane 1 : THP-1 cell lysate at 10 µg
      Lane 2 : human fetal liver lysate

      Performed under reducing conditions.

      Predicted band size : 110 kDa
    • ab108310, at 1/250, staining CD13 in human kidney tissue by immunohistochemistry.
    • ab108310 showing positive staining in Normal liver tissue.

    • ab108310 showing positive staining in Hepatocellular carcinoma tissue.

    • ab108310 showing positive staining in Astrocytoma tissue.

    • ab108310 showing positive staining in Normal breast tissue.

    • ab108310 showing positive staining in Prostatic carcinoma tissue.

    • ab108310 showing positive staining in Normal tonsil tissue.

    • ab108310 showing positive staining in Normal stomach tissue.

    • ab108310 showing positive staining in Normal colon tissue.

    • ab108310 showing positive staining in Lung adenocarcinoma tissue.

    Anti-CD13 antibody [EPR4058] (ab108310)参考文献

    This product has been referenced in:
    • Cui SX  et al. 13F-1, a novel 5-fluorouracil prodrug containing an Asn-Gly-Arg (NO2) COOCH3 tripeptide, inhibits human colonic carcinoma growth by targeting Aminopeptidase N (APN/CD13). Eur J Pharmacol 734:50-9 (2014). Read more (PubMed: 24726845) »
    • Härdtner C  et al. High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic ß-cells. Int J Mol Med 32:795-804 (2013). WB ; Rat . Read more (PubMed: 23942780) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Pig Cell (brain pericytes)
    Specification brain pericytes
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Paraformaldehyde

    Mrs. Verena Ledwig

    Verified customer

    提交于 Mar 09 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Normal kidney)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization No
    Specification Normal kidney
    Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3%
    Fixative Formaldehyde

    Abcam user community

    Verified customer

    提交于 Feb 24 2016

    Thank you or your inquiry. I am happy to confirm that ab108310 (Anti-CD13 antibody [EPR4058]) is tested and guaranteed for ICC (Immunocytochemistry, which includes cell smears) and IHC-P with Mouse, Rat and Human samples. This antibody has not yet been...

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    Thank you for contacting us. Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing...

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