为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Spleen dendritic cells (Mouse)
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
Our Abpromise guarantee covers the use of ab33483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20808846|
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.
Abcam recommends using acetone fixation.
|IP||Use at an assay dependent concentration.|
Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.
Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"