Anti-CD11b/c抗体[OX42] (ab1211)

概述

  • 产品名称Anti-CD11b/c抗体[OX42]
    参阅全部 CD11b/c 一抗
  • 描述
    小鼠单克隆抗体[OX42] to CD11b/c
  • 特异性Recognises a common epitope shared by CD11b/c and precipitates three polypeptides of Mw160kD, 103kD and 95kD; a fainter band may also be seen at 133kD (PMID: 3512425). It reacts with all macrophages in the peritoneal cavity, activated macrophages and Kupffer cells, but with only 35% of alveolar macrophages. It also binds to all granulocytes and to dendritic cells, cells with microglial morphology in brain, sparse thymic cortical cells and epidermal Langerhans cells. It inhibits complement mediated rosettes.
  • 经测试应用适用于: IHC-FoFr, ELISA, Flow Cyt, IHC-Fr, IHC-FrFl, ICC/IF, IPmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Rat
    不与反应: Mouse
  • 免疫原

    Rat macrophages

  • 阳性对照
    • IF/ICC: NR8383 cells
  • 常规说明

    PMID: 7868861. The antigen is temperature sensitive; we may recommend following the conditions as recommended in this publication. As per publication low temperature paraffin, using 0.5 to 2% paraformaldehyde fixation instead of 4% or using periodate-lysine-paraformaldehyde (PLP) + 0.25% PFA and 0.1% glutaraldehyde might be helpful in detection. In case of any question please do not hesitate to contact technical team at technical@abcam.com.

    The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b/c equivalent antigen.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b/c equivalent antigen.
  • 克隆单克隆
  • 克隆编号OX42
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.

The use of ABC will enable further dilution of the antibody, see Abreview.

ELISA 1/100 - 1/3200.
Flow Cyt 1/25 - 1/200.

(10 µl per million cells).
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/500.
IHC-FrFl Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 - 10 µg/ml.
IP Use at an assay dependent concentration. PubMed: 3512425
  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
    • 组织特异性Predominantly expressed in monocytes and granulocytes.
    • 疾病相关Systemic lupus erythematosus 6
    • 序列相似性Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • 结构域The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Antigen CD11b (p170) antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen like family member C antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11C antibody
      • Cell surface glycoprotein MAC 1 subunit alpha antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3A antibody
      • Integrin alpha M antibody
      • Integrin alpha X antibody
      • Integrin alpha-M antibody
      • Integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • Integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
      • Integrin, alpha X (complement component 3 receptor 4 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • ITGAX antibody
      • Leu M5, alpha subunit antibody
      • Leukocyte adhesion glycoprotein p150,95 alpha chain antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Leukocyte adhesion receptor p150,95 antibody
      • Leukocyte surface antigen p150,95, alpha subunit antibody
      • MAC 1 antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • MO1A antibody
      • Myeloid membrane antigen, alpha subunit antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • OTTHUMP00000163299 antibody
      • p150 95 integrin alpha chain antibody
      • SLEB6 antibody
      see all

    Anti-CD11b/c antibody [OX42] 图像

    • ab1211 stained NR8383 cells. The cells were 100% methanol fixed for 10 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1211 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • ab1211 staining CD11b/c equivalent in rat spleen tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in formaldehyde, blocked with 10% serum for 30 minutes at 24°C, then incubated with ab1211 at a 1/100 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/1000 dilution.

      See Abreview

    • ab1211 staining CD11 in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with NGS in 0.3% PBS-TritonX-100 for 60 minutes at 24°C. The sample was incubated with primary antibody (1/300 in NGS in 0.3% PBS-TritonX-100) at 4°C for 42 hours. Ab150111 (1/500) was used as the secondary antibody.

      See Abreview

    Anti-CD11b/c antibody [OX42] (ab1211)参考文献

    This product has been referenced in:
    • Qin Y  et al. Lipopolysaccharide Preconditioning Induces an Anti-inflammatory Phenotype in BV2 Microglia. Cell Mol Neurobiol 36:1269-1277 (2016). Read more (PubMed: 27048218) »
    • Ma X  et al. Modulation of tumorigenesis by the pro-inflammatory microRNA miR-301a in mouse models of lung cancer and colorectal cancer. Cell Discov 1:15005 (2015). IHC-P ; Mouse . Read more (PubMed: 27462406) »

    See all 27 Publications for this product

    Product Wall

    Thank you for your enquiry. If our datasheet do not state any specific protocols, then a general protocol, which can be found on our website, will be sufficient for use. However, I managed to search on IHC world for a Microglia Staining Protocol...

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    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Antigen retrieval step None
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Brandon Jun

    Verified customer

    提交于 Dec 17 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Lung)
    Permeabilization No
    Specification Lung
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 May 31 2013

    Thank you for contacting us.

    I have included a copy of the datasheet with this email as well as our notes for this product and the list of publications.

    I hope this information is helpful to you. Please do not hesitate to cont...

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    Thank you very much for your call yesterday and for your patience while I have looked into your enquiry.

    This antibody was not tested in-house on perfused-fixed frozen sections, but the OX42 clone has been shown in the literature to work on t...

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    Vielen Dank für Ihre Anfrage.

    Das herstellende Labor hat die Produktion dieses Klons eingestellt. Ich möchte hiermit bestätigen, dass die Produktqualität nicht in Frage steht.

    Ich freue mich eine Kopie des Datenb...

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    Bitte entschuldigen Sie dieses Missverständnis.

    Ich habe mich gerade mit meiner Kollegin Frau Z besprochen und Sie hat Ihnen in der Tat gestern eine Gutschrift versprochen.

    Ich möchte mich dennoch bedanken für das Aus...

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    Thank you for your enquiry and your interest in our products.

    I can confirm that this antibody (ab52478: Anti-CD11b antibody [EP1345Y]) is raised in rabbit; it has been tested for cross-reactivity only with mouse and human CD11b. It has not b...

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    For ab1211: DISCOUNT CODE: *** For ab31630: DISCOUNT CODE: *** Expiration date: April 22nd, 2012 I am very pleased to hear you would like to accept our offer and test ab1211 and ab31630 on human. This code will give you 1 free primary antibody before t...

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    Thank you for contacting us. We can confirm that ab1211 can be used as Dendritic cell markers. Other suitable markers are CD80, CD83, CD197, ILT7, ILT5, ILT1, ILT2, IFN-alpha2, IL-12, TSLPR, CD123 etc. We have number of antibodies avail...

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