Anti-CD11b抗体[MEM-174] (Phycoerythrin) (ab28101)

概述

  • 产品名称Anti-CD11b抗体[MEM-174] (Phycoerythrin)
    参阅全部 CD11b 一抗
  • 描述
    小鼠单克隆抗体[MEM-174] to CD11b (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human granulocytes

  • 常规说明

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

应用

Our Abpromise guarantee covers the use of ab28101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use 20µl for 106 cells or 100µl of whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
    • 组织特异性Predominantly expressed in monocytes and granulocytes.
    • 疾病相关Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    • 序列相似性Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • 结构域The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Anti-CD11b antibody [MEM-174] (Phycoerythrin) 图像

    • ab28101 staining CD11b in Human peripheral blood cells by Flow Cytometry. The sample was incubated with the primary antibody (1/6) for 30 minutes at 4°C. Gating Strategy: Granulocytes.

      See Abreview

    Anti-CD11b antibody [MEM-174] (Phycoerythrin) (ab28101)参考文献

    ab28101 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Fixation None
    Permeabilization No
    Sample Human Cell (Peripheral blood)
    Specification Peripheral blood
    Gating Strategy Granulocytes
    Preparation Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Username

    Ms. Ina Laura Pieper

    Verified customer

    提交于 Sep 24 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Fixation None
    Permeabilization No
    Sample Cow Cell (Peripheral blood)
    Specification Peripheral blood
    Gating Strategy Granulocytes
    Preparation Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Username

    Ms. Ina Laura Pieper

    Verified customer

    提交于 Sep 24 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Fixation None
    Permeabilization No
    Sample Sheep Cell (Peripheral blood)
    Specification Peripheral blood
    Gating Strategy Granulocytes
    Preparation Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Username

    Ms. Ina Laura Pieper

    Verified customer

    提交于 Sep 24 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"