Anti-CD11b抗体[M1/70] (PE/Cy5®) (ab25533)

概述

  • 产品名称
    Anti-CD11b抗体[M1/70] (PE/Cy5®)
    参阅全部 CD11b 一抗
  • 描述
    大鼠单克隆抗体[M1/70] to CD11b (PE/Cy5®)
  • 偶联物
    PE/Cy5®. Ex: 496nm, Em: 670nm
  • 经测试应用
    适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Dog, Human, Pig, Monkey
  • 免疫原

    Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.

  • 阳性对照
    • Mouse spleen cells.
  • 常规说明
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

应用

Our Abpromise guarantee covers the use of ab25533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • 组织特异性
    Predominantly expressed in monocytes and granulocytes.
  • 疾病相关
    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 序列相似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • 结构域
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

图片

  • Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 µg (solid line) or Rat IgG2b (PE/Cy5®) isotype control at 0.125 µg (dashed line).

  • ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils.

    See Abreview

  • Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.
  • ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
    Gating Strategy: Live Macrophages.

    See Abreview

文献

This product has been referenced in:
  • Chen X  et al. Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut. Sci Rep 6:24152 (2016). Flow Cyt . Read more (PubMed: 27080036) »
  • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Read more (PubMed: 25936350) »

See all 4 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Sheep Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Monocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

提交于 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Human Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Granulocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

提交于 Sep 11 2013

Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Cow Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Monocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

提交于 Aug 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: Ficoll-hypaque gradrient
Sample buffer: PBS
Permeabilization
No
Gating Strategy
lymphocytes
Username

Abcam user community

Verified customer

提交于 Feb 19 2013

Product Anti-CD11b antibody [M1/70] (PE/Cy5®) (ab25533) is purified using a Protein G affinity purification column.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Pig Cell (blood leucocytes)
Specification
blood leucocytes
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
monocytes
Username

Abcam user community

Verified customer

提交于 Dec 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rabbit Cell (blood leucocytes)
Specification
blood leucocytes
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
monocytes
Username

Abcam user community

Verified customer

提交于 Dec 20 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen from immune mouse)
Specification
Spleen from immune mouse
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive macrophages
Username

Abcam user community

Verified customer

提交于 Apr 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Dog Cell (blood leucocytes)
Specification
blood leucocytes
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
monocytes
Username

Abcam user community

Verified customer

提交于 Aug 25 2011

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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