- 数据表
- 具体的参考文献 (29)
- 实验方案
概述
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产品名称Anti-CD11b抗体[EP1345Y]
参阅全部 CD11b 一抗 -
描述兔单克隆抗体[EP1345Y] to CD11b
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宿主Rabbit
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特异性Testing of mouse and rat tissues (brain, spleen, kidney and heart) in WB gave negative results. However, flow cytometry for mouse RAW 264.7 cell line gave positive results. We have not tested any rat samples in flow cytometry. Due to the variability in mouse, we do not list this as a tested species. We welcome any feedback on mouse and rat reactivity.
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经测试应用适用于: ICC/IF, IHC-FoFr, WB, IP, Flow Cyt, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide corresponding to C terminus of human CD11b
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阳性对照
- IHC-P: Human spleen tissue WB: TF1 lysate. THP-1 macrophages, +10 ng/ml LPS. Flow Cytometry: RAW 264.7 cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EP1345Y
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同种型IgG
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Related Products
应用
Our Abpromise guarantee covers the use of ab52478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| WB | 1/20000 - 1/50000. Predicted molecular weight: 128 kDa. | |
| IP | 1/80. | |
| Flow Cyt | 1/20 - 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
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组织特异性Predominantly expressed in monocytes and granulocytes.
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疾病相关Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
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序列相似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 3684 Human
- Omim: 120980 Human
- SwissProt: P11215 Human
- Unigene: 172631 Human
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别名
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
图片
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](http://www.abcam.cn/ps/products/52/ab52478/Images/CD11b-Primary-antibodies-ab52478-1.jpg)
Flow Cytometry-CD11b antibody [EP1345Y](ab52478)Overlay histogram showing RAW 264.7 cells stained with ab52478 (red line). The cells were fixed with methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52478, 1/50 dilution) for 30 minutes at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] (ab52478)](http://www.abcam.cn/ps/products/52/ab52478/Images/ab52478-272023-ab52478gr9008417huspleen1ugDatasheet.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] (ab52478)IHC image of CD11b staining in a formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab52478, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EP1345Y] (ab52478)](http://www.abcam.cn/ps/products/52/ab52478/Images/ab52478-284700-ab52478-icc.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EP1345Y] (ab52478)ab52478 staining CD11b in the THP-1 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody (1/250). ab150077 was used as the secondary antibody (1/1000). Nuclei were stained with DAPI
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] (ab52478)](http://www.abcam.cn/ps/products/52/ab52478/Images/ab52478_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] (ab52478)Immunohistochemical analysis of paraffin-embedded human spleen using ab52478 at a dilution of 1/100. -
![Western blot - CD11b antibody [EP1345Y] (ab52478)](http://www.abcam.cn/ps/products/52/ab52478/Images/ab52478_1.jpg)
Western blot - CD11b antibody [EP1345Y] (ab52478)Anti-CD11b antibody [EP1345Y] (ab52478) at 1/20000 dilution + TF1 lysate at 10 µg
Secondary
goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 128 kDa
Observed band size: 170 kDa (why is the actual band size different from the predicted?) -
![Western blot - Anti-CD11b antibody [EP1345Y] (ab52478)](http://www.abcam.cn/ps/products/52/ab52478/Images/CD11b-Primary-antibodies-ab52478-7.jpg)
Western blot - Anti-CD11b antibody [EP1345Y] (ab52478)This image is courtesy of an anonymous AbreviewAll lanes : Anti-CD11b antibody [EP1345Y] (ab52478) at 1/5000 dilution
Lane 1 : THP-1 monocytes, untreated
Lane 2 : THP-1 monocytes, +10 ng/ml LPS
Lane 3 : THP-1 macrophages, untreated
Lane 4 : THP-1 macrophages, +10 ng/ml LPS
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : IRDye®680-conjugated donkey anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 180 kDa (why is the actual band size different from the predicted?)
Exposure time: 4 minutes
Blocked with 5% milk for 1 hour at 21°C.
文献
This product has been referenced in:
- Hong D et al. Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection. Stem Cell Res Ther 9:49 (2018). Read more (PubMed: 29482598) »
- Hosono M et al. CXCL8 derived from tumor-associated macrophages and esophageal squamous cell carcinomas contributes to tumor progression by promoting migration and invasion of cancer cells. Oncotarget 8:106071-106088 (2017). Read more (PubMed: 29285315) »
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