概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD11b
  • 经测试应用
    适用于: Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow
    预测可用于: Sheep
  • 免疫原

    Synthetic peptide within Mouse CD11b aa 250-350 (internal sequence). The exact sequence is proprietary.
    Database link: P05555

  • 阳性对照
    • WB: RAW 264.7 whole cell lysate.

应用

Our Abpromise guarantee covers the use of ab75476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 2 µg/ml. Detects a band of approximately 160 kDa.

We have mixed reports of results with human samples. Some lots works some doesn't. In case of negative results with human samples, please use mouse RAW 264.7 cell lysates as positive control.

靶标

  • 功能
    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • 组织特异性
    Predominantly expressed in monocytes and granulocytes.
  • 疾病相关
    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • 序列相似性
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • 结构域
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

图片

  • Anti-CD11b antibody (ab75476) at 2 µg/ml + RAW 264.7 whole cell lysate

    Observed band size : ~160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

文献

This product has been referenced in:
  • Wang X  et al. Role of hypoxia-inducible factor-1a in autophagic cell death in microglial cells induced by hypoxia. Mol Med Rep 15:2097-2105 (2017). Read more (PubMed: 28259912) »
  • Nakamura K  et al. Increase in complement iC3b is associated with anti-inflammatory cytokine expression during late pregnancy in mice. PLoS One 12:e0178442 (2017). WB, IHC-Fr ; Mouse . Read more (PubMed: 28542608) »

See all 41 Publications for this product

客户评价及客户问答

We unfortunately cannot provide ab20147 and ab54217 as FITC conjugated antibodies because we do not custom conjugate the antibodies.

We however have the following anti CD90 FITC conjugated antibodies that may be of your interest

ab...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6.0
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 24 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse oral tumor cells (MOC-2 obtained from Uppalu)
Permeabilization
No
Specification
Mouse oral tumor cells (MOC-2 obtained from Uppalu
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Apr 09 2015

Application
Flow Cytometry
Fixation
None, cells were used fresh
Permeabilization
No
Sample
Mouse Cell (Cell from bone marrow)
Specification
Cell from bone marrow
Gating Strategy
Alive cells (DAPI-), blood cells exluded
Preparation
Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

提交于 Feb 24 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
normal horse blocking serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 24°C
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: trypsin (1:3)
Sample
Rat Tissue sections (alveolar bone)
Specification
alveolar bone
Permeabilization
No
Fixative
Formaldehyde
Username

Ms. Dong-Eun Lee

Verified customer

提交于 Nov 14 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
None
Permeabilization
No
Specification
spleen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Methanol
Username

Ms. CM ou

Verified customer

提交于 Jul 23 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain, cerebral ischemia, 12´m cryo sections)
Specification
brain, cerebral ischemia, 12´m cryo sections
Blocking step
5 % serum, 0,25% Triton-X-100, in TBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Mrs. Birte Könnecke

Verified customer

提交于 Apr 13 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver Teratoma)
Specification
Liver Teratoma
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Dr. Shashank Ganatra

Verified customer

提交于 Oct 15 2012

I am very pleased to hear you would like to test ab75476 in IHC-Fr. This code will give you a discount off your next order before the expiration date. You can use it to purchase an IHC-Fr tested antibody, like ab8878. To redeem this offer, please submi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: AR-10 from Biogenex
Blocking step
Power Block from Biogenex + 2% serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: rt°C
Username

Dr. Laura Dutca

Verified customer

提交于 Sep 28 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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