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Our Abpromise guarantee covers the use of ab75476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 160 kDa.
We have mixed reports of results with human samples. Some lots works some doesn't. In case of negative results with human samples, please use mouse RAW 264.7 cell lysates as positive control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"