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Our Abpromise guarantee covers the use of ab62817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 19071955|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.|
ab62817 (2µg/ml) staining of paraffin embedded Human Brain shows staining of microglia following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining.
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