Anti-CD109抗体[B-E47] (Phycoerythrin) (ab27343)

概述

  • 产品名称
    Anti-CD109抗体[B-E47] (Phycoerythrin)
    参阅全部 CD109 一抗
  • 描述
    小鼠单克隆抗体[B-E47] to CD109 (Phycoerythrin)
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human) -Eahy 926 cell line.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: 5% BSA, PBS
  • Concentration information loading...
  • 纯度
    Ion Exchange Chromatography
  • 克隆
    单克隆
  • 克隆编号
    B-E47
  • 骨髓瘤
    x63-Ag8.653
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab27343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. or 100 µl of whole blood.



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Modulates negatively TGFB1 signaling in keratinocytes.
  • 组织特异性
    Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 5 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.
  • 序列相似性
    Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 翻译后修饰
    N-glycosylated.
    2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 150 KDa TGF beta 1 binding protein antibody
    • 150 KDa TGF-beta-1-binding protein antibody
    • 7D1 antibody
    • 8A3 antibody
    • Activated T cell marker CD109 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 antibody
    • CD 109 antibody
    • CD109 antibody
    • CD109 antigen antibody
    • CD109_HUMAN antibody
    • CPAMD7 antibody
    • DKFZp762L1111 antibody
    • E123 antibody
    • FLJ38569 antibody
    • FLJ41966 antibody
    • Gov system alloantigens on platelets antibody
    • p180 antibody
    • Platelet specific Gov antigen antibody
    • Platelet-specific Gov antigen antibody
    • r150 antibody
    • RP11 525G3.1 antibody
    see all

文献

ab27343 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen of immunized rat
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Jan 09 2013

Application
Flow Cytometry
Sample
Human Cell (from buffy coat)
Specification
from buffy coat
Preparation
Cell harvesting/tissue preparation method: after ficoll extraction
Sample buffer: PBS/BSA 1%
Fixation
none
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Jan 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Jan 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Fixation
none
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

提交于 Jan 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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