Anti-CD105抗体[MEM-229] (ab69772)

概述

  • 产品名称Anti-CD105抗体[MEM-229]
    参阅全部 CD105 一抗
  • 描述
    小鼠单克隆抗体[MEM-229] to CD105
  • 经测试应用适用于: IHC-Fr, ICC/IF, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Tissue, cells or virus corresponding to Human CD105.

  • 阳性对照
    • Kg1 human acute myelogenous leukemia cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab69772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/200. Fix with Acetone.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 71 kDa.

靶标

  • 功能Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • 组织特异性Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • 疾病相关Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Anti-CD105 antibody [MEM-229] 图像

  • ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of frozen infarcted porcine heart labelling CD105 (green) with ab69772 at 5µg/mL. DAPI (blue) was used as nuclear counterstain. 

Anti-CD105 antibody [MEM-229] (ab69772)参考文献

ab69772 has not yet been referenced specifically in any publications.

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Thank you for contacting us. I recommend the following isotype controls for each of these antibodies: ab5694 - Rabbit IgG ab27478 Click here (or use the following: http://www.abcam.c...

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