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Our Abpromise guarantee covers the use of ab21224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-226] to CD105 (ab2529). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [MEM-226] to CD105 (ab2529) as Capture.|
|ELISA||Use a concentration of 1 - 15 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|