概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD105
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide from C-terminal residues of Human CD105.

  • 阳性对照
    • Human Fetal Lung and MCF7 cell lysates, Human Fetal Small Intestine tissue

性能

应用

Our Abpromise guarantee covers the use of ab107595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能
    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • 组织特异性
    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • 疾病相关
    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

图片

  • Membrane staining of formalin-fixed paraffin-embedded Human Fetal Small Intestine with ab107595 at a dilution of 1/100.
  • ab107595 staining CD105 in Rat heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-CD105 antibody (ab107595) at 1/500 dilution

    Lane 1 : Human Fetal Lung lysate
    Lane 2 : MCF7 cell lysate


    Predicted band size : 71 kDa

文献

This product has been referenced in:
  • Kawasaki T  et al. Six1 is required for mouse dental follicle cell and human periodontal ligament-derived cell proliferation. Dev Growth Differ N/A:N/A (2016). IF . Read more (PubMed: 27241908) »
  • Chadchan SB  et al. Endoglin (CD105) coordinates the process of endometrial receptivity for embryo implantation. Mol Cell Endocrinol 425:69-83 (2016). Read more (PubMed: 26802878) »

See all 7 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Antigen retrieval step
None
Sample
Human Tissue sections (synovium)
Specification
synovium
Permeabilization
No
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 05 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (synovium)
Specification
synovium
Permeabilization
No
Fixative
Zinc buffered formalin
Username

Mrs. Merissa Olmer

Verified customer

提交于 Aug 01 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Heart Cells)
Specification
Heart Cells
Fixative
Formaldehyde
Permeabilization
Yes - Triton X
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 10 2013

Thank you for getting back to me with your most recent results.

I am sorry to hear that the protocol suggestions have not improved the results. I can understand your frustration and your reluctance to optimise any further. I believe with the ...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided by you will enable us to investigate this case and will provide us wi...

Read More

D'accord, pas de problème. Merci pour cette information.

N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Merci de votre intérêt pour ab107595.

Comme expliqué par téléphone, cet anticorps n’a pas encore été testé en cytométrie en flux et nous n’offrons pas d’éc...

Read More

Thank you for submitting an Abreview of ab107595. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up ...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lymph node)
Specification
lymph node
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

提交于 May 25 2012

DISCOUNT CODE:42XXXXX Expiration date: 20/04/2012 I am very pleased to hear you would like to accept our offer and test ab107595 in Rat in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer,...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册