Anti-CD105抗体[3A9] (ab114052)

概述

  • 产品名称Anti-CD105抗体[3A9]
    参阅全部 CD105 一抗
  • 描述
    小鼠单克隆抗体[3A9] to CD105
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cyt, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant fragment of Human CD105, expressed in E. coli.

  • 阳性对照
    • IHC-P: Human kidney cancer and stomach cancer tissues ICC/IF: HepG2 cells WB: CD105-hIgGFc transfected HEK293 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab114052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 71 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA 1/10000.

靶标

  • 功能Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • 组织特异性Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • 疾病相关Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

Anti-CD105 antibody [3A9] 图像

  • ab114052, at a 1/200 dilution, staining CD105 in paraffin embedded Human kidney cancer tissue by Immunohistochemistry, with DAB staining.
  • ab114052, at a 1/200 dilution, staining CD105 in paraffin embedded Human stomach cancer tissue by Immunohistochemistry, with DAB staining.
  • All lanes : Anti-CD105 antibody [3A9] (ab114052) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : CD105-hIgGFc transfected HEK293 cell lysate


    Predicted band size : 71 kDa
  • ab114052, at a 1/200 dilution, staining CD105 in HepG2 cells by Immunofluorescence (green).
    Blue: DRAQ5 fluorescent DNA dye.
    Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of CD105 in HepG2 cells, using ab114052 at a 1/200 dilution (green) and negative control (purple).
  • ELISA using ab114052.

Anti-CD105 antibody [3A9] (ab114052)参考文献

This product has been referenced in:
  • Faber A  et al. Interaction of a CD44+ head and neck squamous cell carcinoma cell line with a stromal cell-derived factor-1-expressing supportive niche: An in vitro model. Oncol Lett 7:82-86 (2014). ICC/IF . Read more (PubMed: 24348826) »
  • Chen SY  et al. Isolation and characterization of mesenchymal progenitor cells from human orbital adipose tissue. Invest Ophthalmol Vis Sci 55:4842-52 (2014). IF ; Human . Read more (PubMed: 24994870) »

See all 2 Publications for this product

Product Wall

The immunogen used to create this product spans from aa331-567 of human Endoglin (CD105, SwissProt: P17813 ). A BLAST of this sequence shows a 70% possible homology. This is well below our threshold for predicting cross reactivity however as we have no...

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Thank you for contacting us.

Because this antibody has not been purified and is an ascites, the concentration has not been determined. However we estimate that the concentration will be around 5-10 mg/ml.

I hope this information i...

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Thank you for contacting Abcam.

We do have some information that suggests, heat mediated antigen retrieval using citrate buffer would be successful for using ab114052 in IHC-P.

Below is a link some citrate buffer that would be useful ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

提交于 May 24 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"