The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 105-8 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
相关性All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.
Staining of Molt-4 cell line with 0.5 µg of Mouse IgG1 ? Isotype Control Purified (open histogram) or 0.5 µg of ab95587 (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.
Anti-CD1 antibody [SN13 K5-1B8] (ab95587)参考文献
This product has been referenced in:
Lai J et al. Id1 and NF-?B promote the generation of CD133+ and BMI-1+ keratinocytes and the growth of xenograft tumors in mice. Int J Oncol44:1481-9 (2014).
Read more (PubMed: 24572994) »
Giuliani A et al. Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells. Infect Immun69:7461-70 (2001).
Read more (PubMed: 11705921) »