重组
RabMAb

Anti-CCR7抗体[Y59] (ab32527)

概述

性能

应用

Our Abpromise guarantee covers the use of ab32527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).Can be blocked with CCR7 peptide (ab209386).

For unpurified use at 1/5000.

ICC/IF 1/100.

For unpurified use at 1/250.

IP 1/20.

For unpurified use at 1/10.

靶标

  • 功能
    Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • 组织特异性
    Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BLR 2 antibody
    • BLR2 antibody
    • C C chemokine receptor type 7 antibody
    • C C CKR 7 antibody
    • C-C chemokine receptor type 7 antibody
    • C-C CKR-7 antibody
    • CC chemokine receptor 7 antibody
    • CC chemokine receptor type 7 antibody
    • CC CKR 7 antibody
    • CC-CKR-7 antibody
    • CCCKR7 antibody
    • CCR 7 antibody
    • CCR-7 antibody
    • Ccr7 antibody
    • CCR7_HUMAN antibody
    • CD 197 antibody
    • CD197 antibody
    • CD197 antigen antibody
    • CDw197 antibody
    • Chemokine (C C motif) receptor 7 antibody
    • Chemokine (C C) receptor 7 antibody
    • Chemokine C C motif receptor 7 antibody
    • Chemokine C C receptor 7 antibody
    • Chemokine receptor 7 antibody
    • Chemokine receptor 7-like protein antibody
    • CMKBR7 antibody
    • EBI 1 antibody
    • EBI1 antibody
    • Ebi1h antibody
    • EBV Induced G Protein Coupled Receptor 1 antibody
    • EBV-induced G-protein coupled receptor 1 antibody
    • Epstein Barr virus induced G protein coupled receptor antibody
    • Epstein Barr virus induced gene 1 antibody
    • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
    • EVI 1 antibody
    • EVI1 antibody
    • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
    • MGC108519 antibody
    • MIP 3 beta receptor antibody
    • MIP-3 beta receptor antibody
    • MIP3 Beta Receptor antibody
    see all

Anti-CCR7 antibody [Y59] 图像

  • Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling CCR7 with purified ab32527 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CCR7 with unpurified ab32527 at 1/1000. Cells were fixed with 4% PFA fixed (10 min) and permeabilized and blocked with 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody (ab32527, 1µg/ml) overnight at +4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (shown in green). Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

    Lane 1 : COS-1 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : RAW264.7 cell lysate
    Lane 4 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 43 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/10000 dilution (purified)

    Lane 1 : MCF-7 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 43 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-CCR7 antibody [Y59] (ab32527) at 1/5000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : Daudi cell lysate


    Predicted band size : 43 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • ab32527 (purified) at 1/20 immunoprecipitating CCR7 in MCF-7 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/10,000) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Anti-CCR7 antibody [Y59] (ab32527)参考文献

This product has been referenced in:
  • Sonbul SN  et al. Chemokine (C-C motif) receptor 7 (CCR7) associates with the tumour immune microenvironment but not progression in invasive breast carcinoma. J Pathol Clin Res 3:105-114 (2017). WB ; Human . Read more (PubMed: 28451459) »
  • Zhang L  et al. CCL21/CCR7 Axis Contributed to CD133+ Pancreatic Cancer Stem-Like Cell Metastasis via EMT and Erk/NF-?B Pathway. PLoS One 11:e0158529 (2016). Read more (PubMed: 27505247) »

See all 14 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7)
Gel Running Conditions
Reduced Denaturing
Loading amount
7 µg
Specification
MCF-7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 07 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (CT26-WT tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
CT26-WT tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Miss. Sylvie Colin

Verified customer

提交于 Jun 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Sample
Rat Tissue sections (nerve)
Specification
nerve
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 11 2013

Thank you very much for contacting us.

Rabbit monoclonal antibodies have very high affinity for their antigens, so they are often used more diluted than a comparable mouse monoclonal antibody would be. We don't have any data for htis antibody...

Read More

Thanks for the email.

I'm happy to provide the concentration. It's lot specific so when you have a chance please provide the lot number for that vial of ab32527.

Thanks!

Thanks for your email.

According to information I have this product should arrive as a liquid (100ul). I'm sorry to ask a rather silly question here but did you centrifuge the vial prior to opening, in case all the liquid is in the cap?
...

Read More

Thanks for your email.

I hope you've been able to claim your free antibody. If not, please note I have added your discount code to your review for ab32527 so you should have no problem using that code to get your free primary now.

I...

Read More
Application
Flow Cytometry
Sample
Rat Cell (primary cortical cultures)
Specification
primary cortical cultures
Fixation
none
Permeabilization
No
Gating Strategy
live cells (no PI uptake)
Username

Abcam user community

Verified customer

提交于 Aug 25 2012


I am very pleased to hear you would like to accept our offer and test ab32527 in flow cytometry. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF ANTIBODY OFF OF ORDER before the expiration date. To redeem this offer, please submit an A...

Read More

Thank you for your enquiry.

I've looked and have not been able to find any information regarding flow cytometry testing and ab32527. It may be that this note on our datasheet is an error.

If you'd like to test this antibody in flow ...

Read More

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册