Anti-CCR7抗体(ab103404)

概述

  • 产品名称Anti-CCR7抗体
    参阅全部 CCR7 一抗
  • 描述
    兔多克隆抗体to CCR7
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within N terminal amino acids 20-50 of Human CCR7.

  • 阳性对照
    • WB: 293 cell line lysates and Mouse spleen tissue lysates IHC-P: Tonsil tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab103404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.Can be blocked with CCR7 peptide (ab116167).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • 组织特异性Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BLR 2 antibody
    • BLR2 antibody
    • C C chemokine receptor type 7 antibody
    • C C CKR 7 antibody
    • C-C chemokine receptor type 7 antibody
    • C-C CKR-7 antibody
    • CC chemokine receptor 7 antibody
    • CC chemokine receptor type 7 antibody
    • CC CKR 7 antibody
    • CC-CKR-7 antibody
    • CCCKR7 antibody
    • CCR 7 antibody
    • CCR-7 antibody
    • Ccr7 antibody
    • CCR7_HUMAN antibody
    • CD 197 antibody
    • CD197 antibody
    • CD197 antigen antibody
    • CDw197 antibody
    • Chemokine (C C motif) receptor 7 antibody
    • Chemokine (C C) receptor 7 antibody
    • Chemokine C C motif receptor 7 antibody
    • Chemokine C C receptor 7 antibody
    • Chemokine receptor 7 antibody
    • Chemokine receptor 7-like protein antibody
    • CMKBR7 antibody
    • EBI 1 antibody
    • EBI1 antibody
    • Ebi1h antibody
    • EBV Induced G Protein Coupled Receptor 1 antibody
    • EBV-induced G-protein coupled receptor 1 antibody
    • Epstein Barr virus induced G protein coupled receptor antibody
    • Epstein Barr virus induced gene 1 antibody
    • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
    • EVI 1 antibody
    • EVI1 antibody
    • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
    • MGC108519 antibody
    • MIP 3 beta receptor antibody
    • MIP-3 beta receptor antibody
    • MIP3 Beta Receptor antibody
    see all

Anti-CCR7 antibody 图像

  • ICC/IF image of ab103404 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103404 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CCR7 antibody (ab103404) at 1/100 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 43 kDa
  • Immunohistochemical analysis of CCR7 in formalin fixed and paraffin embedded human tonsil tissue, using a 1/50 dilution of ab103404 followed by peroxidase conjugation of the secondary antibody and DAB staining.
  • Anti-CCR7 antibody (ab103404) at 1/100 dilution + Mouse spleen tissue lysates at 35 µg

    Predicted band size : 43 kDa

Anti-CCR7 antibody (ab103404)参考文献

ab103404 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. I am very happy to hear that the protocol changes improved the results. We are happy to help. To improve the results further concerning the weaker band in the buffy coat, I have two recommendations: 1.) Increase to amount of p...

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Thank you for contacting us. I have understood your question that’s why I recommended the correct way of using this peptide. I am sorry I should have explained this in my last email. ab116167 is not a protein it is a synthetic peptide. The length o...

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Thank you for contacting us. ab116167 and ab103404 can be used in combination with each other. ab116167 can be sued as blocking peptide for ab103404. The concentration/ dilutions have to be determined by end user. For guideline purpose please c...

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Thank you for contacting us. I apologize for the delay. The catalogue number of blocking peptide is ab116167. The price is £110 for 100 ug size. I hope this information is helpful to you. Please do not hesitate to contact us if yo...

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Thank you for contacting us. The positive controls for ab95023 are cell lysates of A-431, U2OS, U251 MG, Hela cell etc. Please click the following to see the positive control tissue lysates; http://www.proteinatlas.org/ENSG00000168685 ...

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