Anti-CCR6抗体[MM0066-3L1] (ab93086)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-CCR6抗体[MM0066-3L1]
参阅全部 CCR6 一抗 -
描述小鼠单克隆抗体[MM0066-3L1] to CCR6
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特异性This antibody detects CCR6, but not other CCRs.
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经测试应用适用于: Flow Cyt, IHC-P, IHC-Frmore details
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种属反应性与反应: Human
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免疫原
Tissue, cells or virus corresponding to Human CCR6. 293 cells transfected with Human CCR6.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: PBS -
Concentration information loading... -
纯度Protein G purified
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纯化说明The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered.
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克隆单克隆
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克隆编号MM0066-3L1
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同种型IgG2b
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研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
Our Abpromise guarantee covers the use of ab93086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| AP | Use a concentration of 1 µg/ml. | |
| Flow Cyt | 1/100 - 1/800. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. | |
| IHC-P | Use a concentration of 1 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. |
靶标
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功能Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
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组织特异性Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
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序列相似性Belongs to the G-protein coupled receptor 1 family.
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细胞定位Cell membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 1235 Human
- Omim: 601835 Human
- SwissProt: P51684 Human
- Unigene: 46468 Human
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别名
- BN 1 antibody
- BN1 antibody
- C C chemokine receptor type 6 antibody
see all
Anti-CCR6 antibody [MM0066-3L1] 图像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [MM0066-3L1] (ab93086)](http://www.abcam.cn/ps/products/93/ab93086/Images/ab93086-3-ab93086.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [MM0066-3L1] (ab93086)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CCR6 with ab93086. Tissue was treated with 15µg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
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](http://www.abcam.cn/ps/datasheet/images/93/ab93086/CCR6-Primary-antibodies-ab93086-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-CCR6 antibody [MM0066-3L1](ab93086)IHC image of ab93086 staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93086, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
](http://www.abcam.cn/ps/datasheet/images/93/ab93086/CCR6-Primary-antibodies-ab93086-2.jpg)
Flow Cytometry-Anti-CCR6 antibody [MM0066-3L1](ab93086)Overlay histogram showing Jurkat cells stained with ab93086 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93086, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Anti-CCR6 antibody [MM0066-3L1] (ab93086)参考文献
This product has been referenced in:
- Tang M et al. Expression and Prognostic Significance of Macrophage Inflammatory Protein-3 Alpha and Cystatin A in Nasopharyngeal Carcinoma. Biomed Res Int 2015:617143 (2015). IHC . Read more (PubMed: 26634210) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"