概述

  • 产品名称Anti-CCR6抗体
    参阅全部 CCR6 一抗
  • 描述
    兔多克隆抗体to CCR6
  • 特异性No cross reactivity with other proteins.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human CCR6 aa 358-374 (C terminal). Different from the related rat and mouse sequences by two amino acids
    Sequence:

    RQTSETADNDNASSFTM

性能

应用

Our Abpromise guarantee covers the use of ab78429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.75 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
  • 组织特异性Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BN 1 antibody
    • BN1 antibody
    • C C chemokine receptor type 6 antibody
    • C C CKR 6 antibody
    • C-C chemokine receptor type 6 antibody
    • C-C CKR-6 antibody
    • CC chemokine receptor type 6 antibody
    • CC CKR 6 antibody
    • CC R6 antibody
    • CC-CKR-6 antibody
    • CCCKR6 antibody
    • CCR 6 antibody
    • CCR-6 antibody
    • Ccr6 antibody
    • CCR6_HUMAN antibody
    • CD 196 antibody
    • CD196 antibody
    • CD196 antigen antibody
    • Chemokine (C C motif) receptor 6 antibody
    • Chemokine (C C) receptor 6 antibody
    • Chemokine (CC motif) receptor 6 antibody
    • Chemokine (CC) receptor 6 antibody
    • Chemokine receptor 6 antibody
    • Chemokine receptor like 3 antibody
    • Chemokine receptor-like 3 antibody
    • CKR 6 antibody
    • CKR L3 antibody
    • CKR-L3 antibody
    • CKR6 antibody
    • CKRL3 antibody
    • CMKBR 6 antibody
    • CMKBR6 antibody
    • DCR 2 antibody
    • DCR2 antibody
    • DRY 6 antibody
    • DRY6 antibody
    • G protein coupled receptor 29 antibody
    • G-protein coupled receptor 29 antibody
    • GPR 29 antibody
    • GPR CY4 antibody
    • GPR-CY4 antibody
    • GPR29 antibody
    • GPRCY 4 antibody
    • GPRCY4 antibody
    • LARC receptor antibody
    • Seven transmembrane receptor lymphocyte 22 antibody
    • STRL 22 antibody
    • STRL22 antibody
    see all

Anti-CCR6 antibody 图像

  • ab78429 at 0.5ug/ml staining CCR6 in human large intestine tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

  • All lanes : Anti-CCR6 antibody (ab78429) at 0.75 µg/ml

    Lane 1 : Tissue lysate prepared from Rat Thymus
    Lane 2 : Whole cell lysate prepared from Human MCF7 cells
    Lane 3 : Whole cell lysate prepared from Hela cells
    Lane 4 : Cell lysate prepared from smmc cells

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 42 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-CCR6 antibody (ab78429)参考文献

This product has been referenced in:
  • Chen H  et al. Serpin treatment suppresses inflammatory vascular lesions in temporal artery implants (TAI) from patients with giant cell arteritis. PLoS One 10:e0115482 (2015). Read more (PubMed: 25658487) »
  • Liu J  et al. CCR6 Is a Prognostic Marker for Overall Survival in Patients with Colorectal Cancer, and Its Overexpression Enhances Metastasis In Vivo. PLoS One 9:e101137 (2014). WB ; Mouse . Read more (PubMed: 24979261) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Mammary gland tumor/inflamatory infiltrate)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA
Permeabilization No
Specification Mammary gland tumor/inflamatory infiltrate
Blocking step Sérum and milk (one hour for each one) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 27°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 27 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 5 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Human Tissue sections (tonsil)
Specification tonsil
Permeabilization No
Fixative Formaldehyde
Username

Dr. R Berahovich

Verified customer

提交于 May 09 2014

I will be happy to send the ab1659 to you as a replacement for ab78429 however I did look at the LSBio antibody I think you are talking about, cat #LS-C83958 and they only predict that it will react with mouse tissue based on sequence homology. I have...

Read More

I have looked over all the information that you sent and you have tried all the variations in protocol that I can think of to try and get ab78429 to work in IHC. I would be happy to send you a replacement antibody however I would just like to say that ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"