Anti-CCR6抗体(ab109703)

概述

  • 产品名称Anti-CCR6抗体
    参阅全部 CCR6 一抗
  • 描述
    兔多克隆抗体to CCR6
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CCR6.

  • 阳性对照
    • This antibody gave a positive signal in U937 whole cell lysate as well as both Human Spleen and Human Lymph Node tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal spleen.

性能

应用

Our Abpromise guarantee covers the use of ab109703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 0.5 µg/ml.

靶标

  • 功能Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
  • 组织特异性Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BN 1 antibody
    • BN1 antibody
    • C C chemokine receptor type 6 antibody
    • C C CKR 6 antibody
    • C-C chemokine receptor type 6 antibody
    • C-C CKR-6 antibody
    • CC chemokine receptor type 6 antibody
    • CC CKR 6 antibody
    • CC R6 antibody
    • CC-CKR-6 antibody
    • CCCKR6 antibody
    • CCR 6 antibody
    • CCR-6 antibody
    • Ccr6 antibody
    • CCR6_HUMAN antibody
    • CD 196 antibody
    • CD196 antibody
    • CD196 antigen antibody
    • Chemokine (C C motif) receptor 6 antibody
    • Chemokine (C C) receptor 6 antibody
    • Chemokine (CC motif) receptor 6 antibody
    • Chemokine (CC) receptor 6 antibody
    • Chemokine receptor 6 antibody
    • Chemokine receptor like 3 antibody
    • Chemokine receptor-like 3 antibody
    • CKR 6 antibody
    • CKR L3 antibody
    • CKR-L3 antibody
    • CKR6 antibody
    • CKRL3 antibody
    • CMKBR 6 antibody
    • CMKBR6 antibody
    • DCR 2 antibody
    • DCR2 antibody
    • DRY 6 antibody
    • DRY6 antibody
    • G protein coupled receptor 29 antibody
    • G-protein coupled receptor 29 antibody
    • GPR 29 antibody
    • GPR CY4 antibody
    • GPR-CY4 antibody
    • GPR29 antibody
    • GPRCY 4 antibody
    • GPRCY4 antibody
    • LARC receptor antibody
    • Seven transmembrane receptor lymphocyte 22 antibody
    • STRL 22 antibody
    • STRL22 antibody
    see all

Anti-CCR6 antibody 图像

  • All lanes : Anti-CCR6 antibody (ab109703) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 3 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 2 minutes
  • IHC image of CCR6 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109703, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CCR6 antibody (ab109703)参考文献

ab109703 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (inflamed skin)
Specification inflamed skin
Permeabilization Yes - 0.1% Triton X-100
Fixative Acetone
Username

Dr. R Berahovich

Verified customer

提交于 Aug 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"