概述

  • 产品名称Anti-CCR5抗体
    参阅全部 CCR5 一抗
  • 描述
    山羊多克隆抗体to CCR5
  • 特异性This antibody binds to CCR 5 receptors on human peripheral blood leukocytes as determined by immunocytochemistry.
  • 经测试应用适用于: IHC-Fr, WB, IHC-P, ICCmore details
  • 种属反应性
    与反应: Human, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    YQVSSPIYDINYYTSEPCQKINVKQIAA

    , corresponding to N terminal amino acids 3-30 of Human CCR5.

  • 阳性对照
    • Human peripheral blood cells or paraffin sections of spleen.

性能

应用

Our Abpromise guarantee covers the use of ab1673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent dilution.
WB 1/1000.
IHC-P 1/300. with biotinylated secondary and streptavidin-HRP.
ICC 1/300.

靶标

  • 功能Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
  • 组织特异性Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
  • 疾病相关Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
    O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
    Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
    Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AM4 7 antibody
    • C C chemokine receptor type 5 antibody
    • C C CKR 5 antibody
    • C-C chemokine receptor type 5 antibody
    • C-C CKR-5 antibody
    • C-C motif chemokine receptor 5 A159A antibody
    • CC Chemokine Receptor 5 antibody
    • CC Chemokine Receptor Type 5 antibody
    • CC CKR 5 antibody
    • CC-CKR-5 antibody
    • CCCKR 5 antibody
    • CCCKR5 antibody
    • CCR 5 antibody
    • CCR-5 antibody
    • CCR5 antibody
    • CCR5 chemokine (C C motif) receptor 5 antibody
    • CCR5_HUMAN antibody
    • CD 195 antibody
    • CD195 antibody
    • CD195 Antigen antibody
    • Chemokine C C motif receptor 5 antibody
    • Chemokine receptor CCR5 antibody
    • CHEMR13 antibody
    • CKR 5 antibody
    • CKR5 antibody
    • CMKBR 5 antibody
    • CMKBR5 antibody
    • FLJ78003 antibody
    • HIV 1 Fusion Coreceptor antibody
    • HIV-1 fusion coreceptor antibody
    • HIV1 fusion coreceptor antibody
    • IDDM22 antibody
    • MIP-1 alpha receptor antibody
    see all

Anti-CCR5 antibody 图像

  • Immunohistochemistry using ab1673 on a section of normal human spleen 

Anti-CCR5 antibody (ab1673)参考文献

This product has been referenced in:
  • Zimmermann T  et al. Low expression of chemokine receptor CCR5 in human colorectal cancer correlates with lymphatic dissemination and reduced CD8+ T-cell infiltration. Int J Colorectal Dis 25:417-24 (2010). WB, IHC-P ; Human . Read more (PubMed: 20054600) »
  • Tan MC  et al. Disruption of CCR5-dependent homing of regulatory T cells inhibits tumor growth in a murine model of pancreatic cancer. J Immunol 182:1746-55 (2009). IHC-P ; Human . Read more (PubMed: 19155524) »

See all 4 Publications for this product

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Thank you for contacting us.
I am sorry that the vial you received did not contain the full amount of the product. We are very careful to measure out exactly the stated quantity, however, sometimes during delivery the cap can become loosened and s...

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Thank you for your enquiry. I can confirm that ab1673 recognizes a band of approximately 40kDa. and that CCR5 has a predicted molecular weight of 40524Da. If you are continually obtaining a band of 37KDa may I suggest that you fill in our techn...

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The image supplied shows a paraffin section of normal human spleen tissue. I suspect that the blocking step with the Sniper reagent may be a problem. Here is the protocol that we recommend. Immunohistochemistry on Paraffin sections: Paraff...

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Thank you for your email. I'm not familiar with the universal immunoenzyme polymer method and so I cannot comment as to how it compares with the ABC method. As you are using the Vectastain Universal Quick Kit, I do suggest following their suggestions. ...

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Thank you for the details that you have provided. I have a few more questions for you that will help me to troubleshoot this problem: 1. I was unable to open the attachments that you sent along with your email. You mentioned in your email that "All ...

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