概述

  • 产品名称Anti-CCR4抗体
    参阅全部 CCR4 一抗
  • 描述
    山羊多克隆抗体to CCR4
  • 特异性Peptide sequence is < 50 % identical to other human chemokine receptors in this region. The antibody recognizes mouse CCR4 and has no cross-reactivity to human CCR4.
  • 经测试应用适用于: IHC-Fr, ELISA, ICC, WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    Synthetic peptide:

    DTTQDETVYNSYYFYESMPC

    , corresponding to amino acids 8-26 of Mouse CCR4.

  • 阳性对照
    • Mouse spleen cells

性能

应用

Our Abpromise guarantee covers the use of ab1664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ELISA 1/500000.
ICC 1/500.
WB 1/1000.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

靶标

  • 功能High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
  • 组织特异性Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C C chemokine receptor type 4 antibody
    • C C CKR 4 antibody
    • C-C chemokine receptor type 4 antibody
    • C-C CKR-4 antibody
    • CC CKR 4 antibody
    • CC-CKR-4 antibody
    • CCR 4 antibody
    • CCR-4 antibody
    • CCR4 antibody
    • CCR4_HUMAN antibody
    • CD194 antibody
    • Chemokine (CC motif) receptor 4 antibody
    • chemokine C C motif receptor 4 antibody
    • ChemR13 antibody
    • CKR4 antibody
    • CMKBR 4 antibody
    • CMKBR4 antibody
    • HGCN 14099 antibody
    • K5 5 antibody
    • K5-5 antibody
    • MGC88293 antibody
    see all

Anti-CCR4 antibody (ab1664)参考文献

This product has been referenced in:
  • Richter JR  et al. Macrophage-derived chemokine (CCL22) is a novel mediator of lung inflammation following hemorrhage and resuscitation. Shock 42:525-31 (2014). IHC-P ; Mouse . Read more (PubMed: 25136780) »
  • Poppensieker K  et al. CC chemokine receptor 4 is required for experimental autoimmune encephalomyelitis by regulating GM-CSF and IL-23 production in dendritic cells. Proc Natl Acad Sci U S A 109:3897-902 (2012). Flow Cyt ; Mouse . Read more (PubMed: 22355103) »

See all 13 Publications for this product

Product Wall

Thank you very much for your patience and for the details in which you have provided. I contacted the originator of ab1664 and below is the FACS protocl that they used. I hope this will be of use to you and if you continue to experience difficulty with...

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Thank you very much for your enquiry and patience. At this time, the only publication that we are currently aware of featuring the use of ab1664 is the Hoogewerf A et al. reference. We also don't have any images/figures to share at this time. If you ca...

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Cysteine molecules are commonly placed on the end of an immunising amino acid sequence. The reason for this is two fold: 1) It allows the peptide to be sulphur-linked to a suitable carrier molecule to provoke an immune response in the host animal. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"