概述

  • 产品名称Anti-CCR3抗体[Y31]
    参阅全部 CCR3 一抗
  • 描述
    兔单克隆抗体[Y31] to CCR3
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Macaque Monkey
  • 免疫原

    A synthetic peptide corresponding to residues in C-terminal cytoplasmic domain of human CKR3.

  • 阳性对照
    • K562 and Daudi cell lysates and human liver tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-CCR3 antibody (HRP) [Y31] (ab200020)

性能

应用

Our Abpromise guarantee covers the use of ab32512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/500.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
    • 组织特异性In eosinophils as well as trace amounts in neutrophils and monocytes.
    • 序列相似性Belongs to the G-protein coupled receptor 1 family.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B chemokine receptor antibody
      • b-chemokine receptor antibody
      • C C chemokine receptor type 3 antibody
      • C C CKR 3 antibody
      • C-C chemokine receptor type 3 antibody
      • C-C CKR-3 antibody
      • CC chemokine receptor 3 antibody
      • CC chemokine receptor type 3 antibody
      • CC CKR 3 antibody
      • CC R3 antibody
      • CC-CKR-3 antibody
      • CCCKR3 antibody
      • CCR 3 antibody
      • CCR-3 antibody
      • CCR3 antibody
      • CCR3_HUMAN antibody
      • CD 193 antibody
      • CD193 antibody
      • CD193 antigen antibody
      • Chemokine (C C motif) receptor 3 antibody
      • chemokine (C-C motif) receptor 3 antibody
      • Chemokine (CC) receptor 1 like 2 antibody
      • Chemokine CC motif receptor 3 antibody
      • Chemokine receptor 3 antibody
      • CK R3 antibody
      • CKR 3 antibody
      • CKR3 antibody
      • CMKB R3 antibody
      • CMKBR 3 antibody
      • CMKBR1L2 antibody
      • CMKBR3 antibody
      • Eosinophil CC chemokine receptor 3 antibody
      • Eosinophil eotaxin receptor antibody
      • Eotaxin receptor antibody
      • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
      • MGC102841 antibody
      • MIP1 Alpha RL2 antibody
      see all

    Anti-CCR3 antibody [Y31] 图像

    • Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.
    • All lanes : Anti-CCR3 antibody [Y31] (ab32512) at 1/500 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Daudi cell lysate


      Predicted band size : 43 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    Anti-CCR3 antibody [Y31] (ab32512)参考文献

    This product has been referenced in:
    • Sun RL  et al. CC-Chemokine Ligand 2 (CCL2) Suppresses High Density Lipoprotein (HDL) Internalization and Cholesterol Efflux via CC-Chemokine Receptor 2 (CCR2) Induction and p42/44 Mitogen-activated Protein Kinase (MAPK) Activation in Human Endothelial Cells. J Biol Chem 291:19532-44 (2016). WB ; Human . Read more (PubMed: 27458015) »
    • Orzekowsky-Schroeder R  et al. Probing the immune and healing response of murine intestinal mucosa by time-lapse 2-photon microscopy of laser-induced lesions with real-time dosimetry. Biomed Opt Express 5:3521-40 (2014). Read more (PubMed: 25360369) »

    See all 8 Publications for this product

    Product Wall

    Thank you very much for your reply and for sending this additional information.

    Your credit note ID is ***.

    I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the origina...

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    Thank you very much for contacting us and letting us know about the trouble with these antibodies.

    Thank you also for sending so many details of your protocol.The protocol seems to be ok and similar to the protocol that the lab uses to test t...

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    Thank you for your inquiry.

    In general we recommend to use EDTA pH9. This antigen retrieval method is optimal for the most antigens. Please see the attachment and this publication (Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Jurkat cells)
    Loading amount 100000 cells
    Specification Jurkat cells
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"