概述

  • 产品名称
  • 描述
    兔多克隆抗体to CCR3
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    ab36827 was raised against a 14 amino acid peptide from near the carboxy terminus of CCR3 (Human).

  • 阳性对照
    • Human spleen cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab36827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

靶标

  • 功能
    Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • 组织特异性
    In eosinophils as well as trace amounts in neutrophils and monocytes.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B chemokine receptor antibody
    • b-chemokine receptor antibody
    • C C chemokine receptor type 3 antibody
    • C C CKR 3 antibody
    • C-C chemokine receptor type 3 antibody
    • C-C CKR-3 antibody
    • CC chemokine receptor 3 antibody
    • CC chemokine receptor type 3 antibody
    • CC CKR 3 antibody
    • CC R3 antibody
    • CC-CKR-3 antibody
    • CCCKR3 antibody
    • CCR 3 antibody
    • CCR-3 antibody
    • CCR3 antibody
    • CCR3_HUMAN antibody
    • CD 193 antibody
    • CD193 antibody
    • CD193 antigen antibody
    • Chemokine (C C motif) receptor 3 antibody
    • chemokine (C-C motif) receptor 3 antibody
    • Chemokine (CC) receptor 1 like 2 antibody
    • Chemokine CC motif receptor 3 antibody
    • Chemokine receptor 3 antibody
    • CK R3 antibody
    • CKR 3 antibody
    • CKR3 antibody
    • CMKB R3 antibody
    • CMKBR 3 antibody
    • CMKBR1L2 antibody
    • CMKBR3 antibody
    • Eosinophil CC chemokine receptor 3 antibody
    • Eosinophil eotaxin receptor antibody
    • Eotaxin receptor antibody
    • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
    • MGC102841 antibody
    • MIP1 Alpha RL2 antibody
    see all

Anti-CCR3 antibody 图像

  • Lane 1 : Anti-CCR3 antibody (ab36827) at 1 µg/ml
    Lanes 2 - 3 : Anti-CCR3 antibody (ab36827) at 2 µg/ml

    Lane 1 : Human spleen tissue lysates
    Lane 2 : Human spleen tissue lysates
    Lane 3 : Human spleen tissue lysates with Blocking peptide


    Predicted band size : 43 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab36827 at 10µg/ml staining CCR3 in human spleen tissue by IHC 

  • Immunofluorescence of CCR3 in Human Spleen cells using ab36827 at 20 ug/ml.

Anti-CCR3 antibody (ab36827)参考文献

This product has been referenced in:
  • Lee R  et al. Enhanced chemokine-receptor expression, function, and signaling in healthy African American and scleroderma-patient monocytes are regulated by caveolin-1. Fibrogenesis Tissue Repair 8:11 (2015). IHC . Read more (PubMed: 26322128) »
  • Musante V  et al. RANTES modulates the release of glutamate in human neocortex. J Neurosci 28:12231-40 (2008). Read more (PubMed: 19020017) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
Permeabilization
No
Specification
Spleen
Blocking step
Vector Casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Henry King

Verified customer

提交于 Aug 18 2017

Vielen Dank für Ihre Anfrage.

Könnten Sie mir bitte noch mitteilen,mitwelchenCCR3 Polyclonal Antibody Sie den ab36827 vergleichen möchten, da wir eine sehr große Auswahl an polyklonalen Antikörpern haben?

Ich...

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Thank you for your reply.

I am happy to confirm that ab36827 is tested and guaranteed by our Abpromise to work in WB with human, mouse and rat samples.

http://www.abcam.com/abpromise

I can suggest to run a positive control...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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