Anti-CCR3抗体(ab36827)
Key features and details
- Rabbit polyclonal to CCR3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CCR3抗体
参阅全部 CCR3 一抗 -
描述
兔多克隆抗体to CCR3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
ab36827 was raised against a 14 amino acid peptide from near the carboxy terminus of CCR3 (Human).
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阳性对照
- IHC-P:Human spleen tissue. ICC/IF: Human spleen cells WB: Human spleen cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36827于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa).
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IHC-P | (2) |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 43 kDa). |
IHC-P
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
靶标
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功能
Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
组织特异性
In eosinophils as well as trace amounts in neutrophils and monocytes. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1232 Human
- Omim: 601268 Human
- SwissProt: P51677 Human
- Unigene: 506190 Human
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别名
- B chemokine receptor antibody
- b-chemokine receptor antibody
- C C chemokine receptor type 3 antibody
see all
图片
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ab36827 at 10µg/ml staining CCR3 in human spleen tissue by IHC
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Lane 1 : Anti-CCR3 antibody (ab36827) at 1 µg/ml
Lanes 2-3 : Anti-CCR3 antibody (ab36827) at 2 µg/ml
Lanes 1-2 : Human spleen tissue lysates
Lane 3 : Human spleen tissue lysates with Blocking peptide
Predicted band size: 43 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
Immunofluorescence of CCR3 in Human Spleen cells using ab36827 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab36827 被引用在 4 文献中.
- Xu C et al. High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia. Mucosal Immunol 11:1306-1315 (2018). PubMed: 29867080
- Lee R et al. Enhanced chemokine-receptor expression, function, and signaling in healthy African American and scleroderma-patient monocytes are regulated by caveolin-1. Fibrogenesis Tissue Repair 8:11 (2015). IHC . PubMed: 26322128
- Kuwabara Y et al. Rapid and transient upregulation of CCL11 (eotaxin-1) in mouse ovary during terminal stages of follicular development. Am J Reprod Immunol 67:358-68 (2012). PubMed: 22221885
- Musante V et al. RANTES modulates the release of glutamate in human neocortex. J Neurosci 28:12231-40 (2008). PubMed: 19020017