概述

  • 产品名称Anti-CCR3抗体[5E8]
    参阅全部 CCR3 一抗
  • 描述
    小鼠单克隆抗体[5E8] to CCR3
  • 经测试应用适用于: Flow Cyt, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human) - human CCR3 transfected into mouse B cell lymphoma L1.2 cells.

  • 阳性对照
    • Blood eosinophils and basophils.

性能

应用

Our Abpromise guarantee covers the use of ab16231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 5 µg/ml. In PBMC stains a very small subset of basophis as well as eosinophils.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • 组织特异性In eosinophils as well as trace amounts in neutrophils and monocytes.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B chemokine receptor antibody
    • b-chemokine receptor antibody
    • C C chemokine receptor type 3 antibody
    • C C CKR 3 antibody
    • C-C chemokine receptor type 3 antibody
    • C-C CKR-3 antibody
    • CC chemokine receptor 3 antibody
    • CC chemokine receptor type 3 antibody
    • CC CKR 3 antibody
    • CC R3 antibody
    • CC-CKR-3 antibody
    • CCCKR3 antibody
    • CCR 3 antibody
    • CCR-3 antibody
    • CCR3 antibody
    • CCR3_HUMAN antibody
    • CD 193 antibody
    • CD193 antibody
    • CD193 antigen antibody
    • Chemokine (C C motif) receptor 3 antibody
    • chemokine (C-C motif) receptor 3 antibody
    • Chemokine (CC) receptor 1 like 2 antibody
    • Chemokine CC motif receptor 3 antibody
    • Chemokine receptor 3 antibody
    • CK R3 antibody
    • CKR 3 antibody
    • CKR3 antibody
    • CMKB R3 antibody
    • CMKBR 3 antibody
    • CMKBR1L2 antibody
    • CMKBR3 antibody
    • Eosinophil CC chemokine receptor 3 antibody
    • Eosinophil eotaxin receptor antibody
    • Eotaxin receptor antibody
    • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
    • MGC102841 antibody
    • MIP1 Alpha RL2 antibody
    see all

Anti-CCR3 antibody [5E8] 图像

  • ICC/IF image of ab16231 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16231, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CCR3 antibody [5E8] (ab16231)参考文献

This product has been referenced in:
  • Plewako H  et al. A follow-up study of immunotherapy-treated birch-allergic patients: effect on the expression of chemokines in the nasal mucosa. Clin Exp Allergy 38:1124-31 (2008). Read more (PubMed: 18691293) »

See 1 Publication for this product

Product Wall

You dont need to permiabilise the cells for flow cytometry.

ab16231's immunogen is human CCR3 transfected into mouse B cell lymphoma L1.2 cells. I have performed a sequence alignment between the bovine and human sequences and found a 72% total homology suggesting that there may be cross-reactions with this anti...

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Thank you very much for your interest in our anti-CCR3 antibodies. To our knowledge, none of ouranti-CCR3 antibodieshas not been tested in Flow Cytometry as well as in IHC-P. Therefore, I can offer a discount off a future purchase if you buy an anti-CC...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"