概述

  • 产品名称Anti-CCNYL1抗体
    参阅全部 CCNYL1 一抗
  • 描述
    兔多克隆抗体to CCNYL1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (

    VKCVTLAIYYHIKNRDANRSLDIFDERSHPLTREKVPEEYFKHDPEHKFI

    ) of Human CCNYL1 (NP_689736).

  • 阳性对照
    • Human fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab87161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-CCNYL1 antibody 图像

  • Anti-CCNYL1 antibody (ab87161) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 40 kDa,50 kDa. We are unsure as to the identity of these extra bands.

Anti-CCNYL1 antibody (ab87161)参考文献

This product has been referenced in:
  • Zi Z  et al. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. PLoS Genet 11:e1005485 (2015). WB ; Mouse . Read more (PubMed: 26305884) »

See 1 Publication for this product

Product Wall

Thank you for sending your protocol and the image of your blot. You have already tried most of the protocol modifications I would recommend in this case. The only other suggestion I would make is that it might help to use 5% milk for both the blocking ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"