概述

  • 产品名称Anti-CCNK抗体
    参阅全部 CCNK 一抗
  • 描述
    兔多克隆抗体to CCNK
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 530 and 580 of human CCNK (NP_001092872.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.

性能

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应用

Our Abpromise guarantee covers the use of ab85854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for IP.
  • 靶标

    Anti-CCNK antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) breast carcinoma (center) and ovarian carcinoma (right) tissues labelling CCNK with ab85854 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-CCNK antibody (ab85854) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : Jurkat whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique

      Predicted band size : 66 kDa


      Exposure time : 3 minutes
    • All lanes : Anti-CCNK antibody (ab85854) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 66 kDa
      Observed band size : 64 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • IHC image of CCNK staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85854, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-CCNK antibody (ab85854)参考文献

    ab85854 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Dec 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"