概述

  • 产品名称Anti-CCNB1IP1抗体[4H3]
    参阅全部 CCNB1IP1 一抗
  • 描述
    小鼠单克隆抗体[4H3] to CCNB1IP1
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human CCNB1IP1 produced in E.coli (NP_878269).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY CCNB1IP1 cDNA; HeLa and Jurkat cells.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab118999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 32 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis.
  • 组织特异性Highly expressed in heart. Detected at intermediate levels in liver and kidney, and at low levels in placenta, brain and lung.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 RING-type zinc finger.
  • 翻译后修饰Ubiquitinated; autoubiquitinated.
    Phosphorylated by CDK1 on serine or threonine residues (in vitro).
  • 细胞定位Nucleus. Chromosome. May associate with segregating chromosomes during metaphase and anaphase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C14orf18 antibody
    • CCNB1IP1 antibody
    • Chromosome 14 open reading frame 18 antibody
    • CIP1_HUMAN antibody
    • Cyclin B1 interacting protein 1 antibody
    • cyclin B1 interacting protein 1, E3 ubiquitin protein ligase antibody
    • Cyclin-B1-interacting protein 1 antibody
    • E3 ubiquitin-protein ligase CCNB1IP1 antibody
    • Enhancer of invasion 10 antibody
    • Gm288 antibody
    • HEI10 antibody
    • Human enhancer of invasion 10 antibody
    • mei4 antibody
    see all

Anti-CCNB1IP1 antibody [4H3] 图像

  • All lanes : Anti-CCNB1IP1 antibody [4H3] (ab118999)

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY cotrol cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CCNB1IP1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 32 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202047)
  • ab118999 at 1/100 dilution staining CCNB1IP1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab118999 at 1/100 dilution staining CCNB1IP1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

Anti-CCNB1IP1 antibody [4H3] (ab118999)参考文献

This product has been referenced in:
  • Holloway JK  et al. Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites. J Cell Biol 205:633-41 (2014). Read more (PubMed: 24891606) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Spermatocytes)
Specification Spermatocytes
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 May 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"