概述

  • 产品名称Anti-CCN1抗体[3H3]
    参阅全部 CCN1 一抗
  • 描述
    小鼠单克隆抗体[3H3] to CCN1
  • 经测试应用适用于: ICC/IF, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Recombinant fragment: IKDPMEDQDG LLGKELGFDA SEVELTRNNE LIAVGKGSSL KRLPVFGMEP RILYNPLQGQ KCIVQTTSWS QCSKT, corresponding to amino acids 168-242 of human CCN1 purified from E. coli

  • 阳性对照
    • Jurkat cell lysates.
  • 常规说明


    ab80112 is derived from hybridization of mouse FO myeloma cells with spleen cells from immunized BALB/c mice.
    The isotype is mouse IgG1 heavy chain and kappa light chain.

性能

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应用

Our Abpromise guarantee covers the use of ab80112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/50.
WB 1/250 - 1/1000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
  • 序列相似性Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3CH61 antibody
    • CCN 1 antibody
    • CCN family member 1 antibody
    • CYR 61 antibody
    • Cyr61 antibody
    • CYR61 protein antibody
    • CYR61_HUMAN antibody
    • Cysteine rich angiogenic inducer 61 antibody
    • Cysteine rich heparin binding protein 61 antibody
    • Cysteine-rich angiogenic inducer 61 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • IBP-10 antibody
    • IGF-binding protein 10 antibody
    • Igfbp 10 antibody
    • IGFBP-10 antibody
    • Igfbp10 antibody
    • Insulin like growth factor binding protein 10 antibody
    • Insulin-like growth factor-binding protein 10 antibody
    • Protein CYR61 antibody
    • Protein GIG1 antibody
    see all

Anti-CCN1 antibody [3H3] 图像

  • Anti-CCN1 antibody [3H3] (ab80112) at 1/250 dilution + Jurkat cell lysate at 40 µg

    Secondary
    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab80112 at 1/50 dilution staining CCN1 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) staining technique.

  • ICC/IF image of ab80112 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80112, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CCN1 antibody [3H3] (ab80112)参考文献

This product has been referenced in:
  • Cardoso J  et al. CYR61 and TAZ Upregulation and Focal Epithelial to Mesenchymal Transition May Be Early Predictors of Barrett's Esophagus Malignant Progression. PLoS One 11:e0161967 (2016). IHC-P ; Human . Read more (PubMed: 27583562) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Mouse Cell lysate - whole cell (osteosarcoma)
Specification osteosarcoma
Total protein in input 200 µg
Username

Abcam user community

Verified customer

提交于 Jul 15 2014

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (osteosarcoma)
Specification osteosarcoma
Total protein in input 200 µg
Username

Abcam user community

Verified customer

提交于 Jun 26 2014

Thank you for contacting us.

I confirm the sodium citrate concentration used was 0.1M.

Both 0.1M and 0.01M are fine. In our online protocols we specify using 0.01M concentration for the buffer, but if 0.1M concentration is used inst...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"