The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.5 µg/ml (allows the detection of 0.2-0.4 ng/well of recombinant C10).
WB: Use at a concentration of 0.1 - 0.2 µg/ml (the detection limit for recombinant C10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions). Predicted molecular weight: 13 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.