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Our Abpromise guarantee covers the use of ab77077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 48 kDa).Can be blocked with CCKBR peptide (ab208978). The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022).|
|IHC-P||Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab77077 (4µg/ml) staining of paraffin embedded Human Pancreas tissue. Steamed antigen retrieval carried using Tris/EDTA buffer pH 9 followed by HRP-staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"