Anti-CCK2-R抗体(ab77077)
Key features and details
- Goat polyclonal to CCK2-R
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CCK2-R抗体
参阅全部 CCK2-R 一抗 -
描述
山羊多克隆抗体to CCK2-R -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow -
免疫原
Synthetic peptide corresponding to Human CCK2-R aa 251-266.
Sequence:C-FDGDSDSDSQSRVRNQ
(Peptide available asab208978) -
阳性对照
- IHC-P: Human pancreas WB: Mouse pancreas tissue lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab77077 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77077于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 6 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 48 kDa.
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说明 |
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IHC-P
Use a concentration of 6 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 48 kDa. |
靶标
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功能
Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism. -
组织特异性
Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC28 cell lines, not expressed in GLC19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 281665 Cow
- Entrez Gene: 887 Human
- Entrez Gene: 12426 Mouse
- Omim: 118445 Human
- SwissProt: P79266 Cow
- SwissProt: P32239 Human
- SwissProt: P56481 Mouse
- Unigene: 203 Human
see all -
别名
- CCK B antibody
- CCK B receptor antibody
- CCK BR antibody
see all
图片
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Anti-CCK2-R antibody (ab77077) at 0.5 µg/ml + Mouse Pancreas lysate at 35 µg
Predicted band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling CCK2-R with ab77077 at 6µg/mL. Negative Control: no primary antibody.
Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
数据表及文件
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Datasheet download
文献 (9)
ab77077 被引用在 9 文献中.
- Smith JP et al. Gastrin Vaccine Alone and in Combination With an Immune Checkpoint Antibody Inhibits Growth and Metastases of Gastric Cancer. Front Oncol 11:788875 (2021). PubMed: 34926305
- Smith JP et al. Cholecystokinin-B Receptor-Targeted Nanoparticle for Imaging and Detection of Precancerous Lesions in the Pancreas. Biomolecules 11:N/A (2021). PubMed: 34944412
- Grzmil M et al. Pharmacological inhibition of mTORC1 increases CCKBR-specific tumor uptake of radiolabeled minigastrin analogue [177Lu]Lu-PP-F11N. Theranostics 10:10861-10873 (2020). PubMed: 33042258
- Matsuda T et al. Distinct CCK-positive SFO neurons are involved in persistent or transient suppression of water intake. Nat Commun 11:5692 (2020). PubMed: 33173030
- Osborne N et al. Vaccine against gastrin, a polyclonal antibody stimulator, decreases pancreatic cancer metastases. Am J Physiol Gastrointest Liver Physiol 317:G682-G693 (2019). PubMed: 31433212
- Smith JP et al. Cholecystokinin receptor antagonist alters pancreatic cancer microenvironment and increases efficacy of immune checkpoint antibody therapy in mice. Cancer Immunol Immunother 67:195-207 (2018). ICC/IF . PubMed: 29043413
- Smith JP et al. Cholecystokinin receptor antagonist halts progression of pancreatic cancer precursor lesions and fibrosis in mice. Pancreas 43:1050-9 (2014). PubMed: 25058882
- Xu L et al. Heterobivalent ligands target cell-surface receptor combinations in vivo. Proc Natl Acad Sci U S A : (2012). IHC-Fr . PubMed: 23236171
- Berna MJ et al. CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production. J Biol Chem 285:38905-14 (2010). ICC/IF ; Rat . PubMed: 20843811