概述

  • 产品名称
  • 描述
    兔多克隆抗体to CCKBR
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Cow, Dog
  • 免疫原

    Poly Lysin conjugated synthetic peptide:

    VHRWPSARVRQTWS

    , corresponding to amino acids 206-219 of Human CCKBR.

性能

应用

Our Abpromise guarantee covers the use of ab14440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000. Predicted molecular weight: 50 kDa.
IHC-P 1/100.

靶标

  • 功能
    Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
  • 组织特异性
    Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC28 cell lines, not expressed in GLC19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCK B antibody
    • CCK B receptor antibody
    • CCK BR antibody
    • CCK-B receptor antibody
    • CCK-BR antibody
    • CCK2 R antibody
    • CCK2 receptor antibody
    • CCK2-R antibody
    • CCKB antibody
    • CCKB receptor antibody
    • CCKBR antibody
    • CCKR Type B antibody
    • CCKRB antibody
    • Cholecystokinin 2 receptor antibody
    • Cholecystokinin B receptor antibody
    • Cholecystokinin-2 receptor antibody
    • Cholecystokinin2 receptor antibody
    • CholecystokininB receptor antibody
    • GASR antibody
    • GASR_HUMAN antibody
    • Gastrin receptor antibody
    • Gastrin/cholecystokinin type B receptor antibody
    • Gastrin\cholecystokinin brain receptor antibody
    see all

文献

This product has been referenced in:
  • Sellam F  et al. Immunohistochemical examination of cholecystokinin and gastrin receptors (CCK-2/gastrin-R) expression in normal and exocrine cancerous human pancreatic tissues. Pancreatology N/A:N/A (2015). IHC-P, IHC-P ; Human . Read more (PubMed: 26520651) »

See 1 Publication for this product

客户评价及客户问答


The pretreatment note in the protocol of the IHC kit refers to the antigen retrieval step that is required for most antibody to achieve a successful IHC-P staining.
For both of these antibodies (ab14440 and ab52894) I can recommend to use Sod...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Guinea pig Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 03 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 03 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Cat Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 03 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Sep 12 2013

Thank you for your enquiry. As this antibody was originated outside of this company, we are unable to provide you with the peptide used as the immunogen. Unfortunately we don't have further information about this antibody. We apologize for the inconven...

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Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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