概述

  • 产品名称Anti-CCK4抗体
    参阅全部 CCK4 一抗
  • 描述
    兔多克隆抗体to CCK4
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 15 amino acid peptide from near the amino terminus of human CCK4.

  • 阳性对照
    • Human, mouse or rat colon membrane lysate

性能

应用

Our Abpromise guarantee covers the use of ab62074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 118 kDa.
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr 1/500.

靶标

  • 功能Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
  • 组织特异性Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 结构域The protein kinase domain is predicted to be catalytically inactive.
  • 翻译后修饰MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
  • 细胞定位Membrane. Cell junction. Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 8430404F20Rik antibody
    • CCK 4 antibody
    • CCK-4 antibody
    • Colon carcinoma kinase 4 antibody
    • Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody
    • Inactive tyrosine-protein kinase 7 antibody
    • mPTK7/CCK4 antibody
    • OTTHUMP00000217016 antibody
    • Protein tyrosine kinase PTK 7 antibody
    • Protein-tyrosine kinase 7 antibody
    • Pseudo tyrosine kinase receptor 7 antibody
    • PTK 7 antibody
    • PTK7 antibody
    • PTK7 protein tyrosine kinase 7 antibody
    • PTK7 protein tyrosine kinase antibody
    • PTK7_HUMAN antibody
    • Serum response factor antibody
    • Srf antibody
    • Tyrosine-protein kinase-like 7 antibody
    see all

Anti-CCK4 antibody 图像

  • ab62074 staining CCK4 in E9.5 mouse embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 5% donkey serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    Image shows sagittal section of E9.5 mouse embryo. Neuroectoderm and underlying mesoderm labelled with ab62074 in green. DAPI stained nuclei in blue.

    See Abreview

  • All lanes : Anti-CCK4 antibody (ab62074) at 1 µg/ml

    Lane 1 : Human colon tissue lysate
    Lane 2 : Mouse colon tissue lysate
    Lane 3 : Rat colon tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    anti-rabbit IgG

    Predicted band size : 118 kDa
  • ab62074 at 5 µg/ml staining CCK4 in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

Anti-CCK4 antibody (ab62074)参考文献

ab62074 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (E9.5 mouse embryo section)
Specification E9.5 mouse embryo section
Permeabilization Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Fixative Paraformaldehyde
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提交于 Aug 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"