Anti-CCK4抗体(ab117412)

概述

  • 产品名称Anti-CCK4抗体
    参阅全部 CCK4 一抗
  • 描述
    兔多克隆抗体to CCK4
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Purified Human CCK4 protein, emulsified in complete adjuvant.

  • 阳性对照
    • MCF7 and T4D7 cells extracts.

性能

应用

Our Abpromise guarantee covers the use of ab117412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 133 kDa (predicted molecular weight: 93 kDa).
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
  • 组织特异性Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 结构域The protein kinase domain is predicted to be catalytically inactive.
  • 翻译后修饰MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
  • 细胞定位Membrane. Cell junction. Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 8430404F20Rik antibody
    • CCK 4 antibody
    • CCK-4 antibody
    • Colon carcinoma kinase 4 antibody
    • Human colon carcinoma kinase 4 (CCK4) mRNA complete cds antibody
    • Inactive tyrosine-protein kinase 7 antibody
    • mPTK7/CCK4 antibody
    • OTTHUMP00000217016 antibody
    • Protein tyrosine kinase PTK 7 antibody
    • Protein-tyrosine kinase 7 antibody
    • Pseudo tyrosine kinase receptor 7 antibody
    • PTK 7 antibody
    • PTK7 antibody
    • PTK7 protein tyrosine kinase 7 antibody
    • PTK7 protein tyrosine kinase antibody
    • PTK7_HUMAN antibody
    • Serum response factor antibody
    • Srf antibody
    • Tyrosine-protein kinase-like 7 antibody
    see all

Anti-CCK4 antibody 图像

  • ab117412 staining CCK4 (green) in wild type mouse embryonic stem cells (D3) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1), permeabilized with 0.1% Triton X-100 and blocked with 1% serum for 30 minutes at 16°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 2 hour at 16°C. ab15007, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) - nuclei.

    See Abreview

  • All lanes : Anti-CCK4 antibody (ab117412) at 1/500 dilution

    Lane 1 : MCF7 cells extracts
    Lane 2 : T4D7 cells extracts


    Predicted band size : 93 kDa

Anti-CCK4 antibody (ab117412)参考文献

ab117412 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization Yes - see blocking buffer
Specification undifferentiated Human ES cells on MEFs
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
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提交于 Feb 23 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HUES7 ES cells)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification HUES7 ES cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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提交于 Feb 04 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEPG2)
Specification HEPG2
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
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提交于 Nov 18 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 20 µg
Specification HEPG2
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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提交于 Nov 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (small intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization No
Specification small intestine
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered Formalin
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提交于 Jul 27 2015

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
Specification Mouse embryonic stem cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Verified customer

提交于 Sep 23 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample Mouse Cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Permeabilization Yes - 0.1% triton X
Fixative methanol:acetone 1:1
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提交于 Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"