Anti-CCDC90A抗体(ab86335)

概述

  • 产品名称
    Anti-CCDC90A抗体
    参阅全部 CCDC90A 一抗
  • 描述
    兔多克隆抗体to CCDC90A
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cat, Dog
  • 免疫原

    Synthetic peptide from within residues ELHQLKQQVM DEVIKVRTDT KLDFNLEKSR VKELYSLNEK KLLELRTEIV, corresponding to internal sequence amino acids 252-301 of Human CCDC90A (NP_001026883)

  • 阳性对照
    • Human fetal brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab86335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-CCDC90A antibody (ab86335) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 40 kDa

实验方案

文献

ab86335 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Rat Tissue lysate - whole (Brain tissue homogenate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
Brain tissue homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Aug 19 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (4-12& BIS-TRIS)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 20 2014

The immunogen identity with the mouse protein is 92%.

The immunogen has 92% identity with the rat protein, so although cross-reactivity with rat CCDC90A is likely, there is a chance that most of the reactivity with the human protein is due to recognition of an epitope that is not present on the rat protei...

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Gracias por contactarnos.

La homología de secuencias entre el inmunógeno del anticuerpo y la proteína en rata es del 92%. Por tanto, es muy probable que el anticuerpo de buenos resultados en esta especie, en las aplicacio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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