amino acids 249-347 (KAEETHSSLS QELQARLQTV TREKEELLQL SIERGKVLQN KQAEICQLEE KLEIANEDRK HALERFEQEA VAVDSNLRVR ELQRKVDGIQ KAYDELRLQ) of the Human CCDC89 protein (Q8N998).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the CCDC89 family.
Cytoplasm. Nucleus. Uniformly distributed within the cell, but becomes recruited to the nucleus upon binding to HEY1.
Information by UniProt
- Bc8 orange-interacting protein antibody
- BOIP antibody
- CCD89_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-CCDC89 antibody (ab122550)
Immunofluorescent staining of Human cell line U-251 MG shows no positivity. Recommended concentration of ab122550 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC89 antibody (ab122550)
ab122550, at 1:200, staining CCDC89 in Human pancreas tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Western blot - Anti-CCDC89 antibody (ab122550)
All lanes : Anti-CCDC89 antibody (ab122550) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal proprietary tag (~3.1 kDa) in mammalian HEK293T cells
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"