Recombinant protein fragment, corresponding to a region within amino acids 17-276 of Human CCDC83 (AAH40280).
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates for WB; HeLa cells for IF/ICC
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 49 kDa.
||1/100 - 1/200.
There are three named isoforms.
- Coiled coil domain containing 83 antibody
- Coiled coil domain containing protein 83 antibody
- HSD9 antibody
Western blot - CCDC83 antibody (ab97917)
Anti-CCDC83 antibody (ab97917) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - CCDC83 antibody (ab97917)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97917 at 1/200 dilution. Lower image is merged with DNA probe.
has not yet been referenced specifically in any publications.
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