概述

  • 产品名称Anti-CCDC77抗体
  • 描述
    兔多克隆抗体to CCDC77
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 401-485 (IMTKRYEALE RRRILEVEGF KTDIKVLRQK LKDLEQMLYK ATVNARANQD LALLCEVRDS NRRAHKIQGE LKNLKSKVFG LENEL) of Human CCDC77.

  • 阳性对照
    • Human lateral ventricle tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-CCDC77 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nuclear membrane. Recommended concentration of ab122513 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122513 at 1/250 dilution staining CCDC77 in Paraffin-embedded Human lateral ventricle tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

Anti-CCDC77 antibody (ab122513)参考文献

ab122513 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122513.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"