antigen sequence: SSVLTPKEQE GIQKNNLEEL TEELTKVMVD YIGMENFWKR YNKVKLEQLS LQHRRAQLLD INGKLREMLK QYLDGISVSD EVLSQ, corresponding to amino acids 368-452 of Human CCDC65 (Q8IXS2).
- Human prostate tissue. Human Plasma lysate. Human tonsil tissue lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Highly expressed in adult testis, in spermatocytes and spermatids. Also observed in spermatogonia. Not detected in Leydig cells, nor in fetal testis (at protein level).
Information by UniProt
- CCD65_HUMAN antibody
- CCDC65 antibody
- Coiled coil domain containing 65 antibody
Immunocytochemistry/ Immunofluorescence - Anti-CCDC65 antibody (ab122482)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab122482 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC65 antibody (ab122482)
ab122482 at 1/200 dilution staining CCDC65 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
Western blot - Anti-CCDC65 antibody (ab122482)
All lanes : Anti-CCDC65 antibody (ab122482) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"