antigen sequence: EEKEVEVAKL QDEISLQQAE LQSLREELQR QKELRAQEDP GEALHSALSD RDEAVNKALE LSLQLNRVSL ERDSLSRELL, corresponding to internal sequence amino acids 357-436 of Human CCDC64B.
Human urinary bladder tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/100 - 1/250. Predicted molecular weight: 57 kDa.
Anti-CCDC64B antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-CCDC64B antibody (ab122842)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and cytoskeleton (actin filaments). Recommended concentration of ab122842 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-CCDC64B antibody (ab122842)
All lanes : Anti-CCDC64B antibody (ab122842) at 1/100 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma Lane 4 : Human liver Lane 5 : Human tonsil Predicted band size : 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC64B antibody (ab122842)
ab122842 at 1/2500 dilution staining CCDC64B in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
Anti-CCDC64B antibody (ab122842)参考文献
has not yet been referenced specifically in any publications.
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