internal sequence amino acids 92-191 (DEEDVDEDAH DSEAKVASLR GMELQGCAST QVESENNQEE QKQVRLPESR LTPWEVWFIG KEKEERDRLQ LKALEELNQQ LEKRKEMEER EKRKIIAEEK) of the Human CCDC34 protein (Q96HJ3).
IHC-P: Human cerebral cortex tissue
WB: RT4 and U251 MG cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Note=A chromosomal aberration involving CCDC34 is found in a patient with hamartoma of the retinal pigment epithelium and retina. Translocation t(11;18) (p13;p11.2).
Information by UniProt
Coiled-coil domain-containing protein 34 antibody
Immunocytochemistry/ Immunofluorescence - Anti-CCDC34 antibody (ab122396)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus, nucleoli and nuclear membrane. Recommended concentration of ab122396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-CCDC34 antibody (ab122396)
All lanes : Anti-CCDC34 antibody (ab122396) at 1/250 dilution Lane 1 : RT4 cell lysate Lane 2 : U251 MG cell lysate Lane 3 : Human Plasma cell lysate Lane 4 : Liver cell lysate Lane 5 : Tonsil cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC34 antibody (ab122396)
, at 1/500, staining CCDC34 in paraffin embedded Human cerebral cortex tissue by immunohistochemistry.
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