概述

  • 产品名称Anti-CCDC23抗体
  • 描述
    兔多克隆抗体to CCDC23
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen: DPPARKEKTK VKESVSRVEK AKQKSAQQEL KQRQRAEIYA LNRVMTELEQ QQFDEFCKQM QPPGE, corresponding to amino acids 2-66 of Human CCDC23 (Q8N300).

  • 阳性对照
    • Human esophagus tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

Anti-CCDC23 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121282 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human esophagus tissue labelled with ab121282 at 1/40 dilution.
  • All lanes : Anti-CCDC23 antibody (ab121282) at 1/250 dilution

    Lane 1 : Marker
    Lane 2 : Vector transfected HEK293T cell lysate
    Lane 3 : HEK293T cell lysate transfected with CCDC23 and C-terminal myc-DDK tag

Anti-CCDC23 antibody (ab121282)参考文献

ab121282 has not yet been referenced specifically in any publications.

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