概述

  • 产品名称
  • 描述
    兔多克隆抗体to CCDC21
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 675-725 of Human CCDC21 (NP_073615.2).

  • 阳性对照
    • Jurkat, HeLa and 293T whole cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab117782 was affinity purified using an epitope specific to CCDC21 immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 序列相似性
    Belongs to the CEP85 family.
  • 细胞定位
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Nucleus > nucleolus. Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCDC21 antibody
    • Centrosomal protein of 85 kDa antibody
    • CEP85 antibody
    • CEP85_HUMAN antibody
    • Coiled-coil domain containing 21 antibody
    • Coiled-coil domain-containing protein 21 antibody
    • DKFZp434L0117 antibody
    • DKFZp434P232 antibody
    • FLJ13976 antibody
    • FLJ22000 antibody
    • OTTHUMP00000003454 antibody
    • OTTHUMP00000003455 antibody
    • OTTHUMP00000003456 antibody
    • OTTHUMP00000003457 antibody
    see all

图片

  • All lanes : Anti-CCDC21 antibody (ab117782) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : Jurkat whole cell lysates at 50 µg
    Lane 4 : HeLa whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 86 kDa


    Exposure time : 3 minutes
  • Detection of CCDC21 by Western Blot of Immunprecipitate.
    ab117782 at 1µg/ml staining CCDC21 in 293T whole cell lysate immunoprecipitated using ab117782 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

文献

ab117782 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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