Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 675-725 of Human CCDC21 (NP_073615.2).
Jurkat, HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
ab117782 was affinity purified using an epitope specific to CCDC21 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
Use at 2-10 µg/mg of lysate.
Belongs to the CEP85 family.
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Nucleus > nucleolus. Localizes to centrosomes and nucleolus in interphase. Upon entry into mitosis, relocates from nucleolus and accumulates at spindle poles.
Information by UniProt
Centrosomal protein of 85 kDa antibody
Western blot - Anti-CCDC21 antibody (ab117782)
All lanes : Anti-CCDC21 antibody (ab117782) at 0.1 µg/ml Lane 1 : 293T whole cell lysates at 50 µg Lane 2 : 293T whole cell lysates at 15 µg Lane 3 : Jurkat whole cell lysates at 50 µg Lane 4 : HeLa whole cell lysates at 50 µg Developed using the ECL technique Predicted band size : 86 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-CCDC21 antibody (ab117782)
Detection of CCDC21 by Western Blot of Immunprecipitate.
ab117782 at 1µg/ml staining CCDC21 in 293T whole cell lysate immunoprecipitated using ab117782 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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