antigen sequence: DKNKPFSRVQKVKFKNPLFVLMEEEEQKQLHFEGLQDILPEAQDYFLEAQ GDLLETQGDLTGIQ SVKPDTQAVEMKVQVT, corresponding to amino acids 291-370 of Human CCDC15 (Q0P6D6).
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Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
Coiled-coil domain containing 15 antibody
Immunocytochemistry/ Immunofluorescence - Anti-CCDC15 antibody (ab122651)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm and microtubule organizing center. Recommended concentration of ab122651 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC15 antibody (ab122651)
ab122651, at 1/200, staining CCDC15 in paraffin embedded Human colon tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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