概述

  • 产品名称
    Anti-CCDC114抗体
    参阅全部 CCDC114 一抗
  • 描述
    兔多克隆抗体to CCDC114
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: SKDDQHLLQE QQQKVLQQRM DKVHSEAERL EARFQDVRGQ LEKLKADIQL LFTKAHCDSS MIDDLLGVKT SMGDRDMGLF LSLIEKRLVE, corresponding to amino acids of Human CCDC114.

  • 阳性对照
    • Human rectum tissue; RT 4, U 251 MG and Human tonsil lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

图片

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and cytoplasm. Recommended concentration of ab122798 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-CCDC114 antibody (ab122798) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab122798, at 1/500 dilution, staining CCDC114 in Paraffin-embedded Human rectum tissue by Immunohistochemistry.

文献

ab122798 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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